protein

Recombinant Calprotectin (CALPRO)

MBS2097578

0.01 mg

150 USD

Recombinant Protein

Recombinant Calprotectin (CALPRO)

[Calprotectin (CALPRO)]

[MRP-8/MRP-14; S100A8/A9]

[protein S100-A8 isoform b; Protein S100-A8; protein S100-A8; S100 calcium binding protein A8; Calgranulin-A; Calprotectin L1L subunit; Cystic fibrosis antigen; CFAG; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; S100 calcium-binding protein A8; Urinary stone protein band A]

[CALPRO]

[S100A8; S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; CAGA; CFAG; MRP8; CFAG; MRP-8; p8]

E Coli

> 95%

20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Original Concentration: 200 ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.)

SDS-Page

Sequence

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Met1~Glu93 and Met1~Pro114 co-expression. Tags: N-terminal His Tag. Subcellular Location: Secreted. Traits: Freeze-dried powder. Predicted isoelectric point: 6.0. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

984290540

NP_001306126.1

NM_001319197.1

P05109

Predicted Molecular Mass: 12/14kDa. Accurate Molecular Mass: 14/16kDa as determined by SDS-PAGE reducing conditions.

Antimicrobial Peptides Pathway (1457777); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); Metal Sequestration By Antimicrobial Proteins Pathway (1457778); Neutrophil Degranulation Pathway (1457780); Regulation Of TLR By Endogenous Ligand Pathway (1427857); Toll-Like Receptors Cascades Pathway (1269204)

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family. Chromosomal Location of Human Ortholog: 1q21.3. Cellular Component: cytoskeleton; cytosol; extracellular region; extracellular space; intermediate filament cytoskeleton; nucleus; plasma membrane. Molecular Function: arachidonic acid binding; calcium ion binding; microtubule binding; protein binding; RAGE receptor binding; zinc ion binding. Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; acute inflammatory response; antimicrobial humoral response; apoptosis; astrocyte development; autophagy; chemokine production; chronic inflammatory response; cytokine production; defense response to bacterium; defense response to fungus; inflammatory response; innate immune response; leukocyte migration involved in inflammatory response; neutrophil chemotaxis; neutrophil degranulation; peptidyl-cysteine S-nitrosylation; positive regulation of cell growth; positive regulation of inflammatory response; positive regulation of NF-kappaB transcription factor activity; positive regulation of peptide secretion; regulation of cytoskeleton organization; response to ethanol; response to lipopolysaccharide; response to zinc ion; sequestering of zinc ion; toll-like receptor signaling pathway; wound healing

0.01 mg

150 USD

0.05 mg

290

0.1 mg

460

0.2 mg

565

0.5 mg

1095

1 mg

1630