Active Interleukin 2 Receptor Beta (IL2Rb) | MyBioSource MBS2097343 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Active Interleukin 2 Receptor Beta (IL2Rb)

catalog :

MBS2097343

quantity :

0.01 mg

price :

210 USD

more info or order :

MyBioSource product webpage

image

image 1 :

IL2RB (interleukin-2 receptor subunit beta) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, which binds and responds to a cytokine called IL-2. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant mouse IL2RB with recombinant mouse IL2. Briefly, IL2RB were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL2RB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL2RB with IL2 was shown in Figure 1 and this effect was in a dose dependent manner.

image 2 :

product information

catalog number :

MBS2097343

products type :

Active Protein

products full name :

Active Interleukin 2 Receptor Beta (IL2Rb)

products short name :

[Interleukin 2 Receptor Beta (IL2Rb)]

products name syn :

[CD122; IL2R-b; IL2-RB; P70-75; High affinity IL-2 receptor subunit beta; Interleukin-2 receptor subunit beta; IL-2 receptor subunit beta]

other names :

[interleukin-2 receptor subunit beta; Interleukin-2 receptor subunit beta; interleukin-2 receptor subunit beta; interleukin 2 receptor, beta chain; High affinity IL-2 receptor subunit beta; p70-75; CD_antigen: CD122]

products gene name :

[IL2Rb]

other gene names :

[Il2rb; Il2rb; p70; CD122; IL15Rbeta; Il-2Rbeta; IL-15Rbeta; Il-2/15Rbeta; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB]

host :

E Coli

sequence length :

539

purity :

> 92%

form :

20mM Tris, 150mM NaCI, (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Cell culture, Activity Assays, In vivo assays. May be suitable for use in other assays to be determined by end user).

image1 heading :

Activity Data

image2 heading :

SDS-Page

other info1 :

Tag: N-terminal His Tag. Source: Prokaryotic expression. Usage: Reconsitute in 20mM Tris, 150 mM NaCI (pH 8.0) to a concentration of 0.1-1.0 mg/mL). Do not vortex.

other info2 :

Residues: Ala27~Glu240. Isoelectric Point: 8.1. Predicted Molecular Weight: 26.3kDa. Accurate Molecular Weight: 27kDa

products categories :

CD & Adhesion molecule; Infection immunity

ncbi gi num :

6680427

ncbi acc num :

NP_032394.1

ncbi gb acc num :

NM_008368.4

uniprot acc num :

P16297

ncbi pathways :

ARMS-mediated Activation Pathway (1366978); Axon Guidance Pathway (1366709); Cytokine Signaling In Immune System Pathway (1367586); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (1367572); DAP12 Signaling Pathway (1367573); Developmental Biology Pathway (1366708); Downstream Signal Transduction Pathway (1366988); Downstream Signaling Of Activated FGFR1 Pathway (1366902)

ncbi summary :

The interleukin 2 receptor is composed of alpha and beta subunits. The beta subunit encoded by this gene is very homologous to the human beta subunit and also shows structural similarity to other cytokine receptors. [provided by RefSeq, Jul 2008]

uniprot summary :

IL2RB: Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. Interacts with SHB upon interleukin stimulation. Interacts with HTLV-1 accessory protein p12I. Belongs to the type I cytokine receptor family. Type 4 subfamily. Protein type: Membrane protein, integral; Receptor, cytokine. Chromosomal Location of Human Ortholog: 15 E1 15 37.52 cM. Cellular Component: cell surface; external side of plasma membrane; integral component of membrane; membrane. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-2 binding; interleukin-2 receptor activity. Biological Process: cytokine-mediated signaling pathway; natural killer cell activation; negative regulation of apoptosis

size1 :

0.01 mg

price1 :

210 USD

size2 :

0.05 mg

price2 :

470

size3 :

0.1 mg

price3 :

735

size4 :

0.2 mg

price4 :

910

size5 :

0.5 mg

price5 :

1790

size6 :

1 mg

price6 :

2655

more info or order :

MyBioSource product webpage