catalog number :
MBS2093372
products full name :
Biotin-Linked Polyclonal Antibody to Elastase 1, Pancreatic (ELA1)
products short name :
[Elastase 1, Pancreatic (ELA1)]
products name syn :
[PE; CELA1; Chymotrypsin-Like Elastase Family,Member 1]
other names :
[elastase 1, pancreatic; Chymotrypsin-like elastase family member 1; chymotrypsin-like elastase family member 1; chymotrypsin like elastase family member 1; Elastase-1; Pancreatic elastase 1]
products gene name :
[ELA1]
other gene names :
[CELA1; CELA1; ELA1; ELA1]
purity :
Antigen-specific Affinity Chromatography
form :
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
concentration :
0.59 mg/ml
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20°C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
WB; IHC; ICC; IP.
app notes :
Western blotting: 0.5-2 ug/mL;1:300-1200. Immunohistochemistry: 5-20 ug/mL;1:30-120. Immunocytochemistry: 5-20 ug/mL;1:30-120. Optimal working dilutions must be determined by end user
other info1 :
Organism Species: Mus musculus (Mouse). Source: Antibody labeling. Label: Biotin. Traits: Liquid. Immunogen: Recombinant ELA1 (Leu44~Met259) expressed in E.coli.
ncbi acc num :
EAW58189.1
ncbi summary :
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B. [provided by RefSeq, May 2009]
uniprot summary :
CELA1: Acts upon elastin. Belongs to the peptidase S1 family. Elastase subfamily. Protein type: EC 3.4.21.36; Protease; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12q13.13. Cellular Component: extracellular space. Molecular Function: metal ion binding; serine-type endopeptidase activity. Biological Process: exocrine pancreas development; inflammatory response; multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; positive regulation of angiogenesis; positive regulation of transcription from RNA polymerase II promoter; post-embryonic development; proteolysis; regulation of cell differentiation; regulation of cell proliferation; tissue remodeling; Wnt signaling pathway