antibody

Biotin-Linked Monoclonal Antibody to Interleukin 10 (IL10)

MBS2090723

0.1 mg

195 USD

monoclonal

mouse

biotin

[C3]

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Antibody

Biotin-Linked Monoclonal Antibody to Interleukin 10 (IL10)

[Interleukin 10 (IL10)]

[interleukin-10; Interleukin-10; interleukin-10; interleukin 10; Cytokine synthesis inhibitory factor; CSIF]

[IL10]

[IL10; IL10; CSIF; TGIF; GVHDS; IL-10; IL10A; IL-10; CSIF]

Monoclonal

[C3]

Mouse

178

Protein A + Protein G affinity chromatography

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

0.96 mg/ml

Storage: Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP).

Western blotting: 0.5-2 ug/mL;1:480-1900. Immunohistochemistry: 5-20 ug/mL;1:48-190. Immunocytochemistry: 5-20 ug/mL;1:48-190. Optimal working dilutions must be determined by end user.

Organism Species: Rattus norvegicus (Rat). Source: Monoclonal antibody preparation. Label: Biotin. Traits: Liquid. Immunogen: Recombinant IL10 (Ser19~Asn178) expressed in E.coli.

10835141

NP_000563.1

NM_000572.2

P22301

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Asthma Pathway (83120); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533)

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family. Protein type: Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q32.1. Cellular Component: extracellular region; extracellular space. Molecular Function: cytokine activity; growth factor activity; interleukin-10 receptor binding; protein binding. Biological Process: aging; B cell differentiation; B cell proliferation; cell-cell signaling; cytoplasmic sequestering of NF-kappaB; defense response to bacterium; defense response to protozoan; hemopoiesis; inflammatory response; leukocyte chemotaxis; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of cell proliferation; negative regulation of chronic inflammatory response to antigenic stimulus; negative regulation of cytokine secretion involved in immune response; negative regulation of heterotypic cell-cell adhesion; negative regulation of interferon-alpha biosynthetic process; negative regulation of interferon-gamma production; negative regulation of interleukin-1 production; negative regulation of interleukin-12 production; negative regulation of interleukin-18 production; negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; negative regulation of membrane protein ectodomain proteolysis; negative regulation of MHC class II biosynthetic process; negative regulation of mitotic cell cycle; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of T cell proliferation; negative regulation of tumor necrosis factor biosynthetic process; negative regulation of tumor necrosis factor production; positive regulation of B cell apoptotic process; positive regulation of cell cycle; positive regulation of cytokine secretion; positive regulation of endothelial cell proliferation; positive regulation of heterotypic cell-cell adhesion; positive regulation of macrophage activation; positive regulation of MHC class II biosynthetic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; receptor biosynthetic process; regulation of gene expression; regulation of isotype switching; regulation of synapse organization; response to activity; response to drug; response to glucocorticoid stimulus; response to inactivity; response to insulin stimulus; response to molecule of bacterial origin; type 2 immune response. Disease: Graft-versus-host Disease, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To; Rheumatoid Arthritis

0.1 mg

195 USD

0.2 mg

250

1 mg

580