Monoclonal Antibody to Apolipoprotein B (APOB) | MyBioSource MBS2090608 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Monoclonal Antibody to Apolipoprotein B (APOB)

catalog :

MBS2090608

quantity :

0.01 mg

price :

105 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[D7]

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Figure. Western Blot; Sample: Recombinant APOB, Human.

image 2 :

DAB staining on IHC-P;. Samples: Human Liver Tissue;. Primary Ab: 10ug/ml Mouse Anti-Human APOB. Antibody Second Ab: 2ug/mL HRPLinked Caprine Anti-Mouse IgG. Polyclonal Antibody. (Catalog: MBS2086035)

image 3 :

Western Blot; Sample: Human Serum;. Primary Ab: 3ug/ml Mouse Anti-Human APOB Antibody;. Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody. (Catalog: MBS2086035)

product information

catalog number :

MBS2090608

products type :

Antibody

products full name :

Monoclonal Antibody to Apolipoprotein B (APOB)

products short name :

[Apolipoprotein B (APOB)]

products name syn :

[Apo-B; FLDB; Apo B-100; Apo B-48]

other names :

[apolipoprotein B-100; Apolipoprotein B-100; apolipoprotein B-100; apolipoprotein B]

products gene name :

[APOB]

other gene names :

[APOB; APOB; FLDB; LDLCQ4; apoB-48; apoB-100; Apo B-100; Apo B-48]

clonality :

Monoclonal

isotype :

IgG2a Kappa

clone :

[D7]

host :

Mouse

purity :

Protein A + Protein G affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

1mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 24 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

app notes :

Western blotting: 0.5-5ug/mL. Immunohistochemistry: 5-20ug/mL. Immunocytochemistry: 5-20ug/mL. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Western Blot (WB)

other info1 :

Organism Species: Homo sapiens (Human). Source: Monoclonal antibody preparation. Traits: Liquid

other info2 :

Immunogen: Recombinant APOB expressed in E.coli

ncbi gi num :

105990532

ncbi acc num :

NP_000375.2

ncbi gb acc num :

NM_000384.2

uniprot acc num :

P04114

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Cell Surface Interactions At The Vascular Wall Pathway (106062); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); FOXA1 Transcription Factor Network Pathway (137979); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Hemostasis Pathway (106028); LDL-mediated Lipid Transport Pathway (106159)

ncbi summary :

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA- UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

uniprot summary :

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. Protein type: Carrier; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2p24.1. Cellular Component: cell soma; chylomicron; chylomicron remnant; cytoplasm; cytosol; early endosome; endocytic vesicle lumen; endoplasmic reticulum exit site; endoplasmic reticulum lumen; endoplasmic reticulum membrane; endosome membrane; extracellular exosome; extracellular region; extracellular space; intermediate-density lipoprotein particle; intracellular membrane-bound organelle; low-density lipoprotein particle; lysosomal lumen; mature chylomicron; plasma membrane; smooth endoplasmic reticulum; very-low-density lipoprotein particle. Molecular Function: cholesterol transporter activity; heparin binding; low-density lipoprotein receptor binding; phospholipid binding; protein binding. Biological Process: artery morphogenesis; cellular protein metabolic process; cellular response to prostaglandin stimulus; cellular response to tumor necrosis factor; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; chylomicron assembly; chylomicron remnant clearance; chylomicron remodeling; fertilization; in utero embryonic development; leukocyte migration; lipoprotein biosynthetic process; lipoprotein catabolic process; lipoprotein transport; low-density lipoprotein particle clearance; low-density lipoprotein particle remodeling; nervous system development; positive regulation of cholesterol storage; positive regulation of gene expression; positive regulation of lipid storage; positive regulation of macrophage derived foam cell differentiation; post-embryonic development; post-translational protein modification; receptor-mediated endocytosis; regulation of cholesterol biosynthetic process; response to carbohydrate stimulus; response to estradiol; response to lipopolysaccharide; response to selenium ion; response to virus; retinoid metabolic process; sperm motility; spermatogenesis; toll-like receptor signaling pathway; triacylglycerol mobilization; triglyceride catabolic process; very-low-density lipoprotein particle assembly. Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

110

size3 :

0.05 mg

price3 :

145

size4 :

0.1 mg

price4 :

175

size5 :

0.2 mg

price5 :

230

size6 :

1 mg

price6 :

515

more info or order :

MyBioSource product webpage