Monoclonal Antibody to Collagen Type VII (COL7) | MyBioSource MBS2090607 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Monoclonal Antibody to Collagen Type VII (COL7)

catalog :

MBS2090607

quantity :

0.01 mg

price :

105 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[C9]

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot; Sample: Human Serum. Primary Ab: 2 ug/ml. Mouse Anti-Human COL7 Antibody. Second Ab: 0.2 ug/mL HRP-Linked. Caprine Anti-Mouse IgG Polyclonal. Antibody (Catalog: MBS2086035)

image 2 :

Western Blot: Sample: Recombinant COL7, Human.

product information

catalog number :

MBS2090607

products type :

Antibody

products full name :

Monoclonal Antibody to Collagen Type VII (COL7)

products short name :

[Collagen Type VII (COL7)]

products name syn :

[COL7-A1; COL7A1; EBD1; EBDCT; EBR1; Long-chain collagen; Epidermolysis Bullosa,Dystrophic,Dominant And Recessive; Collagen Alpha-1(VII)chain]

other names :

[collagen alpha-1(VII) chain; Collagen alpha-1(VII) chain; collagen alpha-1(VII) chain; collagen type VII alpha 1 chain; Long-chain collagen; LC collagen]

products gene name :

[COL7]

other gene names :

[COL7A1; COL7A1; EBD1; EBR1; EBDCT; NDNC8; LC collagen]

clonality :

Monoclonal

isotype :

IgG1 Kappa

clone :

[C9]

host :

Mouse

purity :

Protein A + Protein G affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin8300, 50% glycerol.

concentration :

1mg/mL

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition

tested application :

Western Blot (WB) Immunohistochemistry (IHC),Immunocytochemistry (ICC), Immunoprecipitation (IP).

app notes :

Western blotting: 0.5-2 ug/mL;1:500-2000 . Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200. Optimal working dilutions must be determined by end user

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

other info1 :

rganism Species: Homo sapiens (Human). Source: Monoclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant COL7 (Pro190~Asp472) expressed in E.coli

ncbi gi num :

4502961

ncbi acc num :

NP_000085.1

ncbi gb acc num :

NM_000094.3

uniprot acc num :

Q02388

ncbi pathways :

Anchoring Fibril Formation Pathway (730307); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Extracellular Matrix Organization Pathway (576262); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)

ncbi summary :

This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq, Jul 2008]

uniprot summary :

COL7A1: the alpha chain of type VII collagen, an extra-cellular basement membrane protein restricted to the zone beneath stratified squamous epithelia. Type VII collagen fibrils are composed of three identical alpha collagen chains. Forms anchoring fibrils, which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. Dystrophic epidermolysis bullosa can result from defects in this protein or an autoimmune response made to type VII collagen. Homotrimer. Interacts with MIA3, facilitating its loading into transport carriers and subsequent secretion. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Extracellular matrix; Inhibitor; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: basement membrane; collagen type VII trimer; COPII-coated ER to Golgi transport vesicle; endoplasmic reticulum lumen; endoplasmic reticulum-Golgi intermediate compartment membrane; extracellular matrix; extracellular region; extracellular space; Golgi membrane. Molecular Function: identical protein binding; protein binding; serine-type endopeptidase inhibitor activity. Biological Process: cell adhesion; collagen catabolic process; COPII vesicle coating; epidermis development; ER to Golgi vesicle-mediated transport; extracellular matrix organization. Disease: Epidermolysis Bullosa Dystrophica, Autosomal Dominant; Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Epidermolysis Bullosa Dystrophica, Pretibial; Epidermolysis Bullosa Pruriginosa; Epidermolysis Bullosa With Congenital Localized Absence Of Skin And Deformity Of Nails; Nail Disorder, Nonsyndromic Congenital, 8; Transient Bullous Dermolysis Of The Newborn

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

110

size3 :

0.05 mg

price3 :

145

size4 :

0.1 mg

price4 :

185

size5 :

0.2 mg

price5 :

230

size6 :

1 mg

price6 :

525

more info or order :

MyBioSource product webpage