catalog number :
MBS2090451
products type :
Lysis Buffer
products full name :
Lysis Buffer
products short name :
[Lysis Buffer]
storage stability :
Store at 4°C for frequent use. Stored at -20°C for one year. Avoid repeated freeze-thaw cycles.
tested application :
SDS-PAGE, Western Blot (WB), Immunoprecipitation (IP), Co-IP, ELISA (EIA)
app notes :
It's applicable on most experiments, see above.
other info1 :
Product List: The product is one of the following: . th {text-align: left;} Name Applications Lysis Buffer 1: Membrane proteins including surface proteins, receptors Lysis Buffer 2: Cytoplasmic proteins, structural proteins, etc. Lysis Buffer 3: Cytoplasmic proteins, nuclear matrix proteins, membrane proteins, etc. Lysis Buffer 4: Nuclear matrix proteins, endosome and other organelles protein In order to avoid protein degradation, it is recommended to add 1-2mM PMSF into the Lysis Buffer before usage. Reagent: th {text-align: left;} Reagent Quantity Usage and Dosage Lysis Buffer 100ml (1x) 10 7 cells/50mg tissues by 1 ml lysis buffer.
other info2 :
Important Notes: 1. Please avoid freeze/thaw cycles, it's suggested to aliquot when receiving. 2. The lysis process is suggested on the ice. 3. There might be salt precipitation in the lysis buffer after long-term storage at 4°C, but it will dissolve after it is placed at room temperature. So this phenomenon is normal, and it would not affect the result of the experiment. 4. Lysis buffer should be chosen according to the characteristic of the samples and experimental purposes. The type of lysis buffer and optimal experimental condition should be determined by customers, and a pilot experiment is suggested.
products description :
Lysis buffer is used to lyse cells under non-denaturing buffer conditions, it consists of surfactant, a protease inhibitor, etc. It’s mainly used for protein extraction from cells and tissues. The four lysis buffer are optimized from traditional lysis buffer. They’re suitable for lysis of cell membrane, cytoplasm and nucleus etc., which will help the release of proteins and do good for later extraction, ELISA, Western Blot, etc.
size1 :
20 ml Lysis Buffer 1
size2 :
20 ml Lysis Buffer 2
size3 :
20 ml Lysis Buffer 3
size4 :
20 ml Lysis Buffer 4
size5 :
100 ml Lysis Buffer 1
size6 :
100 ml Lysis Buffer 2
size7 :
100 ml Lysis Buffer 3
size8 :
100 ml Lysis Buffer 4