ELISA/assay

Erythroferrone (ERFE) ELISA Kit

MBS2088219

24-Strip-Wells

290 USD

ELISA Kit

Erythroferrone (ERFE) ELISA Kit

[Erythroferrone (ERFE)]

[FAM132B; C1QTNF15; CTRP15; Myonectin; Family With Sequence Similarity 132, Member B; Complement C1q tumor necrosis factor-related protein 15]

[erythroferrone; Erythroferrone; erythroferrone; erythroferrone; Complement C1q tumor necrosis factor-related protein 15; Myonectin]

[ERFE]

[ERFE; ERFE; CTRP15; FAM132B; C1QTNF15; C1QTNF15; CTRP15; FAM132B]

Human

354

This assay has high sensitivity and excellent specificity for detection of EPX. No significant cross-reactivity or interference between EPX and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.6-1,000pg/mL. Sensitivity: 6.0pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EPX were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EPX were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Signal transduction

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EPX in mouse serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to EPX. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to EPX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EPX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EPX in the samples is then determined by comparing the O.D. of the samples to the standard curve.

628601881

NP_001278761.1

NM_001291832.1

Q4G0M1

37,279 Da

FAM132B: Belongs to the FAM132 family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2q37.3. Cellular Component: extracellular region. Molecular Function: hormone activity. Biological Process: cellular iron ion homeostasis

24-Strip-Wells

290 USD

48-Strip-Wells

540

96-Strip-Wells

735

5x96-Strip-Wells

2980

10x96-Strip-Wells

5370