ELISA/assay

Terminal Complement Complex C5b-9 (C5b-9) High Sensitive ELISA Kit

MBS2087071

24-Strip-Wells

290 USD

ELISA Kit

Terminal Complement Complex C5b-9 (C5b-9) High Sensitive ELISA Kit

[Terminal Complement Complex C5b-9 (C5b-9)]

[MAC; Membrane Attack Complex]

[C5b-9]

Mouse

This assay has high sensitivity and excellent specificity for detection of C5b-9. No significant cross-reactivity or interference between C5b-9 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.65ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C5b-9 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C5b-9 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Signal transduction; Metabolic pathway; Apoptosis; Infection immunity

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of C5b-9 in mouse serum, plasma and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to C5b-9. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to C5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C5b-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

24-Strip-Wells

290 USD

48-Strip-Wells

550

96-Strip-Wells

745

5x96-Strip-Wells

3020

10x96-Strip-Wells

5450