PE-Linked Polyclonal Antibody to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) | MyBioSource MBS2079288 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PE-Linked Polyclonal Antibody to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3)

catalog :

MBS2079288

quantity :

0.1 mg

price :

265 USD

clonality :

polyclonal

host :

rabbit

conjugate :

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2079288

products type :

Antibody

products full name :

PE-Linked Polyclonal Antibody to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3)

products short name :

[Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3)]

products name syn :

[SPTLC2L; SPT 3; LCB 3; C20orf38; Aminotransferase 2; Serine-palmitoyl-CoA transferase 3; Long chain base biosynthesis protein 3]

other names :

[Serine palmitoyltransferase, long chain base subunit 3; Serine palmitoyltransferase 3; serine palmitoyltransferase 3; serine palmitoyltransferase long chain base subunit 3; Long chain base biosynthesis protein 2b; LCB2b; Long chain base biosynthesis protein 3; LCB 3; Serine-palmitoyl-CoA transferase 3; SPT 3]

products gene name :

[SPTLC3]

other gene names :

[SPTLC3; SPTLC3; LCB3; SPT3; LCB2B; hLCB2b; SPTLC2L; C20orf38; dJ718P11; dJ718P11.1; C20orf38; SPTLC2L; LCB2b; LCB 3; SPT 3]

clonality :

Polyclonal

host :

Rabbit

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

0.20 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycle. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Western Blot (WB),Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP).

app notes :

Western blotting: 0.5-2 ug/mL;1:100-400. Immunohistochemistry: 5-20 ug/mL;1:10-40. Immunocytochemistry: 5-20 ug/mL;1:10-40. Optimal working dilutions must be determined by end user.

other info1 :

Organism Species: Mus musculus (Mouse). Source: Antibody labeling. Label: PE. Traits: Liquid. Immunogen: Recombinant SPTLC3 (Arg80~Ala563) expressed in E.coli.

other info2 :

Unconjugated Antibody: The unconjugated antibody version of this item is also available as catalog #MBS2032263

ncbi gi num :

223462383

ncbi acc num :

AAI50645.1

ncbi pathways :

Ceramide Biosynthesis Pathway (524496); Ceramide Biosynthesis Pathway (468287); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); Sphingolipid Metabolism Pathway (760636); Sphingolipid De Novo Biosynthesis Pathway (1270098); Sphingolipid Metabolism Pathway (82994); Sphingolipid Metabolism Pathway (1270097); Sphingolipid Metabolism Pathway (369)

ncbi summary :

The SPTLC3 gene encodes an isoform of the third subunit of serine palmitoyltransferase (SPT; EC 2.3.1.50), which catalyzes the rate-limiting step of the de novo synthesis of sphingolipids (Hornemann et al., 2006 [PubMed 17023427]). SPT contains 2 main subunits: the common SPTLC1 subunit (MIM 605712) and either SPTLC2 (MIM 605713) or its isoform SPTLC2L (SPTLC3), depending on the tissue in which biosynthesis occurs (Hornemann et al., 2006 [PubMed 17023427]). There are also 2 highly related isoforms of a third subunit, SSSPTA (MIM 613540) and SSSPTB (MIM 610412), that confer acyl-CoA preference of the SPT enzyme and are essential for maximal enzyme activity (Han et al., 2009 [PubMed 19416851]).[supplied by OMIM, Nov 2010]

uniprot summary :

SPTLC3: Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC3-SSSPTA isozyme uses both C14-CoA and C16-CoA as substrates, while the SPTLC1-SPTLC3-SSSPTB has the ability to use a broader range of acyl-CoAs without apparent preference. Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.3.1.50; Endoplasmic reticulum; Membrane protein, integral; Transferase. Chromosomal Location of Human Ortholog: 20p12.1. Cellular Component: endoplasmic reticulum membrane. Molecular Function: serine C-palmitoyltransferase activity. Biological Process: sphingoid biosynthetic process; sphingolipid biosynthetic process

size1 :

0.1 mg

price1 :

265 USD

size2 :

0.2 mg

price2 :

410

size3 :

1 mg

price3 :

985

more info or order :

MyBioSource product webpage