protein

UBE2D2, 1-147aa, Human, E Coli

MBS206740

0.1 mg

375 USD

Recombinant Protein

UBE2D2, 1-147aa, Human, E Coli

[UBE2D2]

[Ubiquitin-conjugating enzyme E2 D2 isoform1; E2 (17)KB2; PUBC1; UBC4; UBC4/5; UBCH4; UBCH5B]

[ubiquitin-conjugating enzyme E2 D2 isoform 1; Ubiquitin-conjugating enzyme E2 D2; ubiquitin-conjugating enzyme E2 D2; ubiquitin conjugating enzyme E2 D2; (E3-independent) E2 ubiquitin-conjugating enzyme D2 (EC:2.3.2.24]

[UBE2D2]

[UBE2D2; UBE2D2; UBC4; PUBC1; UBCH4; UBC4/5; UBCH5B; E2(17)KB2; PUBC1; UBC4; UBC5B; UBCH4; UBCH5B]

E Coli

147

MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM

> 95% by SDS-PAGE

Liquid. In 20mM MES (pH 6.0) containing 50mM NaCl, 1mM DTT

0.5 mg/ml (determined by Absorbance at 280nm)

Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

SDS-Page

Antigen Species: Human

ubiquitination

UBE2D2, also known as Ubiquitin-conjugating enzyme E2 D2, is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. UBE2D2 catalyzes ubiquitination of IkB-alpha in a phosphorylation and SCFB-TRCP dependent manner. Recombinant human UBE2D2 protein was expressed in E Coli and purified by using conventional chromatography techniques.

Ciechanover A., et al. (1994) FASEB J. 8 (2):182-91.; Orian A., et al. (1995) J Biol Chem. 270 (37):21707-14.

4507775

NP_003330

NM_003339.2

P62837

16.7 kDa (147aa) Confirmed by MALDI-TOF

Activated TLR4 Signalling Pathway (1269236); Adaptive Immune System Pathway (1269171); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (1269193); C-type Lectin Receptors (CLRs) Pathway (1269303); CLEC7A (Dectin-1) Signaling Pathway (1269304); Cellular Response To Hypoxia Pathway (1270415); Cellular Responses To Stress Pathway (1270414); Class I MHC Mediated Antigen Processing Presentation Pathway (1269192); Downstream TCR Signaling Pathway (1269176); E3 Ubiquitin Ligases Ubiquitinate Target Proteins Pathway (1470927)

Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.

0.1 mg

375 USD

0.5 mg

925