PPID antibody | MyBioSource MBS206589 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PPID antibody

catalog :

MBS206589

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[AT1B8]

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPID antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: K562 cell lysate Lane 2.: Jurkat cell lysate Lane 3.: HeLa cell lysate Lane 4.: HepG2 cell lysate Lane 5.: A549 cell lysate Lane 6.: MCF7 cell lysate Lane 7.: SK-OV-3 cell lysate Lane 8.: PC3 cell lysate

image 2 :

ICC/IF analysis of PPID in Hep3B cells. The cell was stained with MBS206589 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

product information

catalog number :

MBS206589

products type :

Antibody

products full name :

PPID antibody

products short name :

[PPID]

products name syn :

[Peptidylprolyl isomerase D; PPID; CYPD; CYP-40; Cyclophilin D; 40 kDa peptidyl prolyl cis trans isomerase; 40 kDa peptidyl prolyl cis trans isomerase D; Cyclophilin related protein; Cyclophilin40; CyclophilinD; CYP 40; CYP40; MGC33096.Peptidylprolyl isomerase D; PPIase; PPID; Rotamase.]

other names :

[peptidyl-prolyl cis-trans isomerase D; Peptidyl-prolyl cis-trans isomerase D; peptidyl-prolyl cis-trans isomerase D; peptidylprolyl isomerase D; 40 kDa peptidyl-prolyl cis-trans isomerase; Cyclophilin-40; CYP-40; Cyclophilin-related protein; Rotamase D]

products gene name :

[PPID]

other gene names :

[PPID; PPID; CYPD; CYP-40; CYP40; CYPD; PPIase D; CYP-40]

clonality :

Monoclonal

isotype :

IgG2b, kappa

clone :

[AT1B8]

host :

Mouse

reactivity :

Human

purity :

By protein-A affinity chromatography

form :

Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.

concentration :

1 mg/ml

storage stability :

Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing/thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

app notes :

The antibody has been tested by ELISA, Western Blot analysis, Flow Cytometry and Immunocytochemistry (ICC)/Immunofluorescence (IF) to assure specific and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Antigen Species: Human. Immunogen: Recombinant human PPID (1-370aa) purified from E. coli

other info2 :

Clone Note: Anti-human PPID mAb, clone AT1B8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPID protein.

products categories :

Immunology

products description :

Cyclophilin D (also known as peptidylpropyl isomerase D, PPID) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. This protein can bind to the immunosuppressant cyclosporine A and was known that its overexpression suppresses the apoptosis in cancer cells.

products references :

MachidaK.,et al. (2006) J. Biol. Chem. 281 (20): 14314-20.; Yokoi H.,et al. (1996) Genomics. 35 (3): 448-55.

ncbi gi num :

4826932

ncbi acc num :

NP_005029

ncbi gb acc num :

NM_005038.2

uniprot acc num :

Q08752

ncbi pathways :

C-MYB Transcription Factor Network Pathway (138073)

ncbi summary :

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. [provided by RefSeq, Jul 2008]

uniprot summary :

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Proposed to act as a co-chaperone in HSP90 complexes such as in unligated steroid receptors heterocomplexes. Different co-chaperones seem to compete for association with HSP90 thus establishing distinct HSP90-co-chaperone-receptor complexes with the potential to exert tissue-specific receptor activity control. May have a preference for estrogen receptor complexes and is not found in glucocorticoid receptor complexes. May be involved in cytoplasmic dynein-dependent movement of the receptor from the cytoplasm to the nucleus. May regulate MYB by inhibiting its DNA-binding activity. Involved in regulation of AHR signaling by promoting the formation of the AHR:ARNT dimer; the function is independent of HSP90 but requires the chaperone activity. Involved in regulation of UV radiation-induced apoptosis. Promotes cell viability in anaplastic lymphoma kinase-positive anaplastic large-cell lymphoma (ALK+ ALCL) cell lines. May be involved in hepatitis C virus (HCV) replication and release.

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage