Cd55, 35-362aa, Mouse, His tag, Baculovirus | MyBioSource MBS206320 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Cd55, 35-362aa, Mouse, His tag, Baculovirus

catalog :

MBS206320

quantity :

0.05 mg

price :

310 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS206320

products type :

Recombinant Protein

products full name :

Cd55, 35-362aa, Mouse, His tag, Baculovirus

products short name :

[Cd55]

products name syn :

[Complement decay-accelerating factor; GPI-anchored; Daf; Daf-GPI; Daf1; GPI-DAF; Cd55]

other names :

[complement decay-accelerating factor, GPI-anchored preproprotein; Complement decay-accelerating factor, GPI-anchored; complement decay-accelerating factor, GPI-anchored; CD55 molecule, decay accelerating factor for complement; CD_antigen: CD55]

products gene name :

[Cd55]

other gene names :

[Cd55; Cd55; Daf; Daf1; Daf-GPI; GPI-DAF; Cd55a; Daf; Daf1; DAF-GPI]

host :

Baculovirus

sequence length :

390

sequence :

DCGPPPDIPN ARPILGRHSK FAEQSKVAYS CNNGFKQVPD KSNIVVCLEN GQWSSHETFC EKSCVAPERL SFASLKKEYL NMNFFPVGTI VEYECRPGFR KQPPLPGKAT CLEDLVWSPV AQFCKKKSCP NPKDLDNGHI NIPTGILFGS EINFSCNPGY RLVGVSSTFC SVTGNTVDWD DEFPVCTEIH CPEPPKINNG IMRGESDSYT YSQVVTYSCD KGFILVGNAS IYCTVSKSDV GQWSSPPPRC IEKSKVPTKK PTINVPSTGT PSTPQKPTTE SVPNPGDQPT PQKPSTVKVS ATQHVPVTKT TVRHPIRTST DKGEPNTGLE HHHHHH

purity :

> 95% by SDS-PAGE

form :

Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.

concentration :

0.5 mg/ml (determined by Absorbance at 280nm)

storage stability :

Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

image1 heading :

SDS-Page

other info2 :

Endotoxin Level: < 1.0 EU per 1 ug of protein (determined by LAL method). Predicted N terminal: Asp 35

products categories :

Immunology

products description :

CD55, also known as Complement decay-accelerating factor, GPI-anchored, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. It is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. Recombinant mouse CD55, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

products references :

Lea S., et al. (2002) Biochem Soc Trans. 30:1014-1019.; Wang Y., et al. (2010) J Neuroinflammation. 7:24.

ncbi gi num :

114326522

ncbi acc num :

NP_034146

ncbi gb acc num :

NM_010016.3

uniprot acc num :

Q61475

ncbi mol weight :

36.8kDa (336aa) 40-57kDa (SDS-PAGE under reducing conditions)

ncbi pathways :

Allograft Rejection Pathway (920963); Asparagine N-linked Glycosylation Pathway (1367240); COPI-mediated Anterograde Transport Pathway (1367178); Class B/2 (Secretin Family Receptors) Pathway (1367080); Complement Activation, Classical Pathway (198379); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (1367536); ER To Golgi Anterograde Transport Pathway (1367175)

ncbi summary :

This gene encodes an inhibitor of both the classical and the alternative pathways of complement activation. The encoded preproprotein undergoes post-translational processing to generate a mature polypeptide anchored to the plasma membrane via a glycosylphosphatidylinositol moiety. Erythrocytes from mice deficient in the encoded protein exhibit impaired regulation of complement activation resulting in enhanced complement deposition. Mice lacking the encoded protein exhibit enhanced susceptibility to experimentally induced myasthenia gravis. This gene is located adjacent to a closely related gene on chromosome 1. [provided by RefSeq, Nov 2015]

uniprot summary :

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage.

size1 :

0.05 mg

price1 :

310 USD

size2 :

0.25 mg

price2 :

925

more info or order :

MyBioSource product webpage