protein

Cd14, 16-366aa, Mouse, His tag, Baculovirus

MBS206286

0.05 mg

375 USD

Recombinant Protein

Cd14, 16-366aa, Mouse, His tag, Baculovirus

[Cd14]

[Monocyte differentiation antigen CD14]

[monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14; monocyte differentiation antigen CD14; CD14 antigen; Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14]

[Cd14]

[Cd14; Cd14]

Baculovirus

16-366aa

> 90% by SDS-PAGE

Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.

0.5 mg/ml (determined by Absorbance at 280nm)

Can be stored at 2-8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

SDS-Page

Tag Information: His-tag

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Immunology

Cd14, also known as monocyte differentiation antigen CD14, is a component of the innate immune system. This protein was found expressed in macrophages, neutrophil granulocyte and dendritic cells. It exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol tail (mCD14), the other a soluble form (sCD14). The major function is serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns. Recombinant Mouse Cd14, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Ferrero E., et al. (1990) J Immunol. 45 (1):331-336.; SD Wright., et al. (1990) Science. 249:1431-1433.

6753332

NP_033971

NM_009841.4

P10810

38.3kDa (357aa). 40-57kDa (SDS-PAGE under reducing conditions)

Activated TLR4 Signalling Pathway (1367531); Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Hematopoietic Cell Lineage Pathway (83275); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (1367472); Innate Immune System Pathway (1367502); Legionellosis Pathway (469202); Legionellosis Pathway (469186); MAPK Signaling Pathway (83245)

This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor. [provided by RefSeq, Jul 2014]

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:16148141). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135, PubMed:15895089). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (). Acts as an accessory receptor for M.tuberculosis lipoproteins LprA, LprG and LpqH, in conjunction with coreceptors TLR2 and TLR1. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- ().

0.05 mg

375 USD

0.25 mg

1195