protein

AKR1D1, 1-326aa, Human, E Coli

MBS206240

0.05 mg

310 USD

Recombinant Protein

AKR1D1, 1-326aa, Human, E Coli

[AKR1D1]

[3o5bred; CBAS2; SRD5B1]

[3-oxo-5-beta-steroid 4-dehydrogenase isoform 1; 3-oxo-5-beta-steroid 4-dehydrogenase; 3-oxo-5-beta-steroid 4-dehydrogenase; aldo-keto reductase family 1 member D1; Aldo-keto reductase family 1 member D1; Delta(4)-3-ketosteroid 5-beta-reductase; Delta(4)-3-oxosteroid 5-beta-reductase]

[AKR1D1]

[AKR1D1; AKR1D1; CBAS2; SRD5B1; 3o5bred; SRD5B1]

E Coli

326

MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY

> 95% by SDS-PAGE

Liquid. In 20mM Tris-HCl Buffer (pH 8.5) containing 1mM DTT, 0.1M NaCl, 10% glycerol

1mg/ml (determined by Absorbance at 280nm)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

SDS-Page

Antigen Species: Human

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Metabolism; Signal Transduction

AKR1D1 also known as 3-oxo-5beta-steroid 4-deghydrogenase isoform 1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-betareduction of bile acid intermediates and steroid hormones which carry a delta (4)-3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction. Recombinant AKR1D1 protein was expressed in E Coli and purified by using conventional chromatography techniques.

Moore S A., et al. (1995) J Mol Biol. 249 (1):195-214; Gould S J., et al. (1988) Anal Biochem. 175 (1):5-13

5174695

NP_005980

NP_005980.1

P51857

37.3 kDa (326aa)

Bile Acid And Bile Salt Metabolism Pathway (1270040); Bile Acid Biosynthesis, Cholesterol = Cholate/chenodeoxycholate Pathway (413432); Bile Acid Biosynthesis, Cholesterol = Cholate/chenodeoxycholate Pathway (468297); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); Metapathway Biotransformation (198837); Primary Bile Acid Biosynthesis Pathway (82938); Primary Bile Acid Biosynthesis Pathway (299); Steroid Hormone Biosynthesis Pathway (82940)

The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet. [provided by RefSeq, Jul 2010]

Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.

0.05 mg

310 USD

0.25 mg

720