protein

Erythropoietin, 28-193aa, Human, His tag, Baculovirus (Bioactivity Validated)

MBS206227

0.05 mg

310 USD

Recombinant Protein

Erythropoietin, 28-193aa, Human, His tag, Baculovirus (Bioactivity Validated)

[Erythropoietin]

[EPO; EP; MVCD2]

[erythropoietin; Erythropoietin; erythropoietin; erythropoietin; INN: Epoetin]

[EPO; EPO; EP; MVCD2]

193

APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALRAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDRLEHH HHHH

> 95% by SDS-PAGE

Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.

0.5 mg/ml (determined by Absorbance at 280nm)

Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

SDS-Page

Tag Information: His-tag. Predicted N terminal: Ala28

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method). Biological Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is equal or less than 0.5ng/ml.

Cardiovascular; Angiogenesis

EPO, also known as erythropoietin, is a glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. It is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. This protein can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types. Recombinant human EPO, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Koury, M.J., et al. (2005) Exp. Hematol. 33:1263.; Jelkmann W., et al. (2007) Eur J Haematol. 78:183-205

62240997

NP_000790

NM_000799.3

P01588

19.5kDa (174aa) 28-40KDa (SDS-PAGE under reducing conditions.)

Cellular Response To Hypoxia Pathway (1270415); Cellular Responses To Stress Pathway (1270414); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Differentiation Pathway (1458184); EPO Receptor Signaling Pathway (198882); EPO Signaling Pathway (138051); HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); HIF-2-alpha Transcription Factor Network Pathway (137956)

This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

0.05 mg

310 USD

0.25 mg

925