S100B, 1-92aa, Human, E Coli | MyBioSource MBS206161 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

S100B, 1-92aa, Human, E Coli

catalog :

MBS206161

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

image

image 1 :

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

product information

catalog number :

MBS206161

products type :

Recombinant Protein

products full name :

S100B, 1-92aa, Human, E Coli

products short name :

[S100B]

products name syn :

[NEF; S100; S100-B; S100beta]

other names :

[protein S100-B; Protein S100-B; protein S100-B; S100 calcium binding protein B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B]

products gene name :

[S100B]

other gene names :

[S100B; S100B; NEF; S100; S100-B; S100beta]

host :

E Coli

sequence positions :

[1-92aa]

sequence length :

sequence :

MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDNDGDGECD FQEFMAFVAM VTTACHEFFE HE

purity :

> 95% by SDS-PAGE

form :

Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol

concentration :

1mg/ml (determined by Bradford assay)

storage stability :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

image1 heading :

SDS-PAGE

other info1 :

Endotoxin Level: <1.0 EU per 1 ug of protein (determined by LAL method). Tag: Non-Tagged

products categories :

Cell Cycle

products description :

S100B also known as protein S100-B. S100B is a protein of the S100 protein family. S100 proteins comprise a multigene family of EF-hand calcium binding proteins that engage in multiple functions in response to cellular stress. S100B protein has been implicated in oligodendrocyte progenitor cell (OPC) regeneration in response to demyelinating insult. Also, S100B and other members of S100 proteins are specifically interact with PP5-TPR and inhibited the PP5-Hsp90 interaction. Recombinant Human S100B was expressed in E Coli and purified by using conventional chromatography techniques.

products references :

Gilquin B., et al. (2010) Mol Cell Biol. 30 (11): 2724-36.; Yamaguchi F., et al. (2012) J Biol Chem. 287 (17): 13787-98.

ncbi gi num :

5454034

ncbi acc num :

NP_006263

ncbi gb acc num :

NM_006272.2

uniprot acc num :

P04271

ncbi mol weight :

10.7kDa (92aa)

ncbi pathways :

Activated TLR4 Signalling Pathway (1269236); Advanced Glycosylation Endproduct Receptor Signaling Pathway (1269258); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1269268); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (1269276); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); MyD88 Cascade Initiated On Plasma Membrane Pathway (1269207); MyD88 Dependent Cascade Initiated On Endosome Pathway (1269228); MyD88-independent TLR3/TLR4 Cascade Pathway (1269220); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (1269237)

ncbi summary :

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

uniprot summary :

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage