protein

Apoa1, 25-264aa, Mouse, His tag, E Coli

MBS206112

0.1 mg

310 USD

Recombinant Protein

Apoa1, 25-264aa, Mouse, His tag, E Coli

[Apoa1]

[Apolipoprotein A-I; Alp-1; apo-AI; Apoa-1; apoA-I; Brp-14,Ltw-1; Lvtw-1; Sep-1; Sep-2; Sep2; Apolipoprotein AI]

[apolipoprotein A-I preproprotein; Apolipoprotein A-I; apolipoprotein A-I; apolipoprotein A-I; Apolipoprotein A1]

[Apoa1]

[Apoa1; Apoa1; Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I; Apo-AI; ApoA-I; ProapoA-I]

E Coli

264

MGSSHHHHHH SSGLVPRGSH MGSDEPQSQW DKVKDFANVY VDAVKDSGRD YVSQFESSSL GQQLNLNLLE NWDTLGSTVS QLQERLGPLT RDFWDNLEKE TDWVRQEMNK DLEEVKQKVQ PYLDEFQKKW KEDVELYRQK VAPLGAELQE SARQKLQELQ GRLSPVAEEF RDRMRTHVDS LRTQLAPHSE QMRESLAQRL AELKSNPTLN EYHTRAKTHL KTLGEKARPA LEDLRHSLMP MLETLKTQVQ SVIDKASETL TAQ

> 90% by SDS-PAGE

Liquid, In Phosphate buffered saline (pH 7.4) containing 20% glycerol, 1mM DTT

1mg/ml (determined by Bradford assay)

Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

SDS-Page

Antigen Species: Mouse. Tag Information: His-tag

Cardiovascular

Apoa1 also known as Apolipoprotein A-I, is the major protein component of high density lipoprotein (HDL) in plasma. This protein promotes cholesterol efflux from tissues to the liver for excretion. And it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apoa1 are associated with HDL deficiency including Tangier disease. Recombinant mouse Apoa1, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques

Sontag TJ., et al. (2014) Arterioscler Thromb Vasc Biol, 34 (2), 262-9.

160333304

NP_033822

NM_009692.4

Q00623

30.3kDa (263aa) confirmed by MALDI-TOF

ABC Transporters In Lipid Homeostasis Pathway (1368002); ABC-family Proteins Mediated Transport Pathway (1368001); African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (1367201); Chylomicron-mediated Lipid Transport Pathway (1367662); Fat Digestion And Absorption Pathway (194386); Fat Digestion And Absorption Pathway (194324); HDL-mediated Lipid Transport Pathway (1367663); Hemostasis Pathway (1368135)

This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is the major protein component of plasma high density lipoprotein (HDL). This protein facilitates the removal of cholesterol and other fats from tissues by transporting them to the liver for excretion. This protein is a cofactor for lecithin cholesterolacyltransferase, an enzyme that catalyzes the conversion of free cholesterol to cholesteryl esters. Mutations in this gene in humans causes familial HDL deficiency, Tangier disease and familial visceral amyloidosis. Similar clinical features are exhibited by mice with mutations in this gene. This gene is clustered with three other apolipoprotein genes on chromosome 9. [provided by RefSeq, Dec 2013]

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

0.1 mg

310 USD

0.5 mg

720