antibody

Cy3-Linked Monoclonal Antibody to Vinculin (VCL)

MBS2059753

0.1 mg

295 USD

monoclonal

mouse

Cy3

[C3]

western blot, immunohistochemistry, immunocytochemistry

Antibody

Cy3-Linked Monoclonal Antibody to Vinculin (VCL)

[Vinculin (VCL)]

[vinculin, partial; Vinculin; vinculin; vinculin; Metavinculin; MV]

[VCL]

[VCL; VCL; MV; MVCL; CMD1W; CMH15; HEL114; MV]

Monoclonal

[C3]

Mouse

Human

Protein A/G Affinity Chromatography

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol

200µg/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB; IHC; ICC

Western blotting: 0.5-2µg/mL. Immunohistochemistry: 5-20µg/mL. Immunocytochemistry: 5-20µg/mL. Optimal working dilutions must be determined by end user

Source: Monoclonal antibody preparation. Label: Cy3. Traits: Liquid. Immunogen: Recombinant VCL (Lys1020~Gln1134) expressed in E.coli.

246714

AAB21657.1

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Hemostasis Pathway (106028)

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 10q22.2. Cellular Component: adherens junction; cell-cell adherens junction; costamere; cytoskeleton; cytosol; extracellular region; focal adhesion; intercellular junction; protein complex. Molecular Function: actin binding; alpha-catenin binding; beta-catenin binding; cadherin binding; protein binding; ubiquitin protein ligase binding. Biological Process: apical junction assembly; cell adhesion; cell motility; cell-matrix adhesion; lamellipodium biogenesis; morphogenesis of an epithelium; muscle contraction; negative regulation of cell migration; platelet degranulation. Disease: Cardiomyopathy, Dilated, 1w

0.1 mg

295 USD

0.2 mg

420

1 mg

990