IDO1, 1-403aa, Human, His tag, E Coli | MyBioSource MBS205715 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

IDO1, 1-403aa, Human, His tag, E Coli

catalog :

MBS205715

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS205715

products type :

Recombinant Protein

products full name :

IDO1, 1-403aa, Human, His tag, E Coli

products short name :

[IDO1]

products name syn :

[Indoleamine 2 3-dioxygenase 1 Indoleamine 23-dioxygenase 1; Indoleamine 2 3-dioxygenase 1; Indoleamine 2,3-dioxygenase 1; IDO; IDO-1; INDO]

other names :

[indoleamine 2,3-dioxygenase 1; Indoleamine 2,3-dioxygenase 1; indoleamine 2,3-dioxygenase 1; indoleamine 2,3-dioxygenase 1; Indoleamine-pyrrole 2,3-dioxygenase]

products gene name :

[IDO1]

other gene names :

[IDO1; IDO1; IDO; INDO; IDO-1; IDO; INDO; IDO-1]

host :

E Coli

sequence length :

403

sequence :

MGSSHHHHHH SSGLVPRGSH MGSMAHAMEN SWTISKEYHI DEEVGFALPN PQENLPDFYN DWMFIAKHLP DLIESGQLRE RVEKLNMLSI DHLTDHKSQR LARLVLGCIT MAYVWGKGHG DVRKVLPRNI AVPYCQLSKK LELPPILVYA DCVLANWKKK DPNKPLTYEN MDVLFSFRDG DCSKGFFLVS LLVEIAAASA IKVIPTVFKA MQMQERDTLL KALLEIASCL EKALQVFHQI HDHVNPKAFF SVLRIYLSGW KGNPQLSDGL VYEGFWEDPK EFAGGSAGQS SVFQCFDVLL GIQQTAGGGH AAQFLQDMRR YMPPAHRNFL CSLESNPSVR EFVLSKGDAG LREAYDACVK ALVSLRSYHL QIVTKYILIP ASQQPKENKT SEDPSKLEAK GTGGTDLMNF LKTVRSTTEK SLLKEG

purity :

> 90% by SDS-PAGE

form :

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

image1 heading :

SDS-Page

other info1 :

Antigen Species: Human. Tag Information: His-tag

products categories :

Metabolism

products description :

IDO1 is an indoleamine 2,3-dioxygenase - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, and serotonin. It is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan. Recombinant human IDO1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

products references :

Metz R., et al (2007). Nature Cancer Res. 67:7082-7087; Yuasa H.J., et al (2007). J. Mol. Evol. 65:705-714

ncbi gi num :

4504577

ncbi acc num :

NP_002155

ncbi gb acc num :

NM_002164.5

uniprot acc num :

P14902

ncbi mol weight :

47.7 kDa (426aa), confirmed by MALDI-TOF

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Histidine, Lysine, Phenylalanine, Tyrosine, Proline And Tryptophan Catabolism Pathway (1309221); L-kynurenine Degradation Pathway (907939); L-tryptophan Degradation III (eukaryotic) Pathway (139214); L-tryptophan Degradation XI (mammalian, Via Kynurenine) Pathway (139210); L-tryptophan Degradation To 2-amino-3-carboxymuconate Semialdehyde Pathway (139207); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Amino Acids And Derivatives Pathway (1270158)

ncbi summary :

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]

uniprot summary :

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed:17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed:25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed:25691885). Acts as a suppressor of anti-tumor immunity (PubMed:23103127, PubMed:25157255, PubMed:14502282, PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed:25691885). Protects the fetus from maternal immune rejection (PubMed:25691885).

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage