AIFM1, 98-609aa, Human, His tag, E Coli | MyBioSource MBS205698 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

AIFM1, 98-609aa, Human, His tag, E Coli

catalog :

MBS205698

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS205698

products type :

Recombinant Protein

products full name :

AIFM1, 98-609aa, Human, His tag, E Coli

products short name :

[AIFM1]

products name syn :

[Apoptosis-inducing factor 1 mitochondrial isoform 2 precursor; Apoptosis-inducing factor 1; mitochondrial isoform 2 precursor; AIF; CMTX4; COWCK; COXPD6; PDCD8]

other names :

[apoptosis-inducing factor 1, mitochondrial isoform AIF-exB; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor 1, mitochondrial; apoptosis inducing factor mitochondria associated 1; Programmed cell death protein 8]

products gene name :

[AIFM1]

other gene names :

[AIFM1; AIFM1; AIF; CMT2D; CMTX4; COWCK; DFNX5; NADMR; NAMSD; PDCD8; COXPD6; AIF; PDCD8]

host :

E Coli

sequence length :

609

sequence :

MGSSHHHHHH SSGLVPRGSH MGSEFLGLTP EQKQKKAALS ASEGEEVPQD KAPSHVPFLL IGGGTAAFAA ARSIRARDPG ARVLIVSEDP ELPYMRPPLS KELWFSDDPN VTKTLRFKQW NGKERSIYFQ PPSFYVSAQD LPHIENGGVA VLTGKKVVQL DVRDNMVKLN DGSQITYEKC LIATGGTPRS LSAIDRAGAE VKSRTTLFRK IGDFRSLEKI SREVKSITII GGGFLGSELA CALGRKARAL GTEVIQLFPE KGNMGKILPE YLSNWTMEKV RREGVKVMPN AIVQSVGVSS GKLLIKLKDG RKVETDHIVA AVGLEPNVEL AKTGGLEIDS DFGGFRVNAE LQARSNIWVA GDAACFYDIK LGRRRVEHHD HAVVSGRLAG ENMTGAAKPY WHQSMFWSDL GPDVGYEAIG LVDSSLPTVG VFAKATAQDN PKSATEQSGT GIRSESETES EASEITIPPS TPAVPQAPVQ GEDYGKGVIF YLRDKVVVGI VLWNIFNRMP IARKIIKDGE QHEDLNEVAK LFNIHED

purity :

> 95% by SDS-PAGE

form :

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

image1 heading :

SDS-Page

other info1 :

Antigen Species: Human. Tag Information: His-tag

products categories :

Metabolism

products description :

Apoptosis-inducing factor 1, mitochondrial isoform 2 precursor, also known as AIFM1, is a mitochondrial protein that translocates to the nucleus upon induction of apoptosis. AIFM1 has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIFM1 from mitochondria, but not to block its apoptogenic activity. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Recombinant human AIFM1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

products references :

Zamzami N., et al. (1996) J Exp Med. 183: 1533-1544.; Susin S A., et al. (1996) J Exp Med. 184: 1331-1341.;

ncbi gi num :

22202629

ncbi acc num :

NP_665811

ncbi gb acc num :

NM_145812.2

uniprot acc num :

O95831

ncbi mol weight :

58.5 kDa (537aa)

ncbi pathways :

Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Modulation And Signaling Pathway (198822); Apoptosis Modulation By HSP70 Pathway (198877); Ceramide Signaling Pathway (138023); Nanomaterial Induced Apoptosis Pathway (1458218)

ncbi summary :

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and cognitive disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Aug 2015]

uniprot summary :

Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage