SELPLG, 42-121aa, Human, His tag, E Coli | MyBioSource MBS205693 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

SELPLG, 42-121aa, Human, His tag, E Coli

catalog :

MBS205693

quantity :

0.02 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS205693

products type :

Recombinant Protein

products full name :

SELPLG, 42-121aa, Human, His tag, E Coli

products short name :

SELPLG

products name syn :

P-selectin glycoprotein ligand 1 isoform 2 precursor; CD162; CLA; PSGL-1; PSGL1

other names :

P-selectin glycoprotein ligand 1 isoform 2; P-selectin glycoprotein ligand 1; P-selectin glycoprotein ligand 1; cutaneous lymphocyte-associated associated antigen; selectin P ligand; Selectin P ligand; CD_antigen: CD162

products gene name :

SELPLG

other gene names :

SELPLG; SELPLG; CLA; CD162; PSGL1; PSGL-1; PSGL-1

uniprot entry name :

SELPL_HUMAN

sequence length :

412

sequence :

MGSSHHHHHH SSGLVPRGSH MGSQATEYEY LDYDFLPETE PPEMLRNSTD TTPLTGPGTP ESTTVEPAAR RSTGLDAGGA VTELTTELAN MGNLSTDSAA MEI

purity :

> 80% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT.

concentration :

0.25 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Cadiovascular

products description :

SELPLG is a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants. Recombinant human SELPLG protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Stubke K, Wicklein D, et al. (2012). Cancer Lett. 321(1):89-99. Gong L, Cai Y, et al. (2012). Pathol Oncol Res. 18(4):989-96.

ncbi gi num :

331284236

ncbi acc num :

NP_002997

ncbi gb acc num :

NM_003006.4

ncbi mol weight :

10.9 kDa (103aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); Staphylococcus Aureus Infection Pathway (172846); Staphylococcus Aureus Infection Pathway (171867); Amb2 Integrin Signaling Pathway (137945)

ncbi summary :

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

uniprot summary :

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 12q24. Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane. Molecular Function: protein binding; receptor binding. Biological Process: cell adhesion; leukocyte tethering or rolling; blood coagulation; leukocyte adhesive activation; leukocyte migration

size1 :

0.02 mg

price1 :

310 USD

size2 :

0.1 mg

price2 :

720

more info or order :

MyBioSource product webpage