HERC5, 681-1024aa, Human, His tag, E Coli | MyBioSource MBS205588 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

HERC5, 681-1024aa, Human, His tag, E Coli

catalog :

MBS205588

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS205588

products type :

Recombinant Protein

products full name :

HERC5, 681-1024aa, Human, His tag, E Coli

products short name :

HERC5

products name syn :

E3 ISG15-protein ligase HERC5; CEB1; CEBP1

other names :

E3 ISG15--protein ligase HERC5; E3 ISG15--protein ligase HERC5; E3 ISG15--protein ligase HERC5; HECT domain and RCC1-like domain-containing protein 5; cyclin-E-binding protein 1; hect domain and RLD 5; probable E3 ubiquitin-protein ligase HERC5; HECT and RLD domain containing E3 ubiquitin protein ligase 5; Cyclin-E-binding protein 1; HECT domain and RCC1-like domain-containing protein 5

products gene name :

HERC5

other gene names :

HERC5; HERC5; CEB1; CEBP1; CEB1; CEBP1

uniprot entry name :

HERC5_HUMAN

sequence length :

1024

sequence :

MGSSHHHHHH SSGLVPRGSH MGSFDLTVRR NHLIEDVLNQ LSQFENEDLR KELWVSFSGE IGYDLGGVKK EFFYCLFAEM IQPEYGMFMY PEGASCMWFP VKPKFEKKRY FFFGVLCGLS LFNCNVANLP FPLALFKKLL DQMPSLEDLK ELSPDLGKNL QTLLDDEGDN FEEVFYIHFN VHWDRNDTNL IPNGSSITVN QTNKRDYVSK YINYIFNDSV KAVYEEFRRG FYKMCDEDII KLFHPEELKD VIVGNTDYDW KTFEKNARYE PGYNSSHPTI VMFWKAFHKL TLEEKKKFLV FLTGTDRLQM KDLNNMKITF CCPESWNERD PIRALTCFSV LFLPKYSTME TVEEALQEAI NNNRGFG

purity :

> 90% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Immunology

products description :

HERC5 is a member of the HERC family of ubiquitin ligases. It is a protein with a HECT domain and five RCC1 repeats. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. HERC5 gene lies in a cluster of HERC family genes on chromosome 4. It has been shown to exhibit antiviral activity towards HIV-1, influenza A virus and human papillomavirus. Recombinant human HERC5 protein, fused to His-tag at N-terminus, was expressed in E.coli.

products references :

Woods MW, Kelly JN, et al. (2011). Retrovirology. 8:95. Tang Y, Zhong G, et al. (2010). J Immunol. 184(10):5777-90.

ncbi gi num :

110825982

ncbi acc num :

NP_057407

ncbi gb acc num :

NM_016323.3

ncbi mol weight :

43 kDa (367aa)

ncbi pathways :

Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Cytokine Signaling In Immune System Pathway (366171); ISG15 Antiviral Mechanism Pathway (530761); Immune System Pathway (106386); Innate Immune System Pathway (106387); Interferon Signaling Pathway (187103); Negative Regulators Of RIG-I/MDA5 Signaling Pathway (187102); RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways (187097)

ncbi summary :

This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq, Jul 2008]

uniprot summary :

HERC5: Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Makes part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant- negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15. Protein type: Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-. Chromosomal Location of Human Ortholog: 4q22.1. Cellular Component: perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus. Molecular Function: protein binding; ISG15 conjugating enzyme activity; ubiquitin-protein ligase activity; ligase activity. Biological Process: ISG15-protein conjugation; cytokine and chemokine mediated signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of defense response to virus; innate immune response; regulation of cyclin-dependent protein kinase activity; defense response to virus; negative regulation of interferon type I production

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage