protein

MMP2, 110-660aa, Human, His tag, E Coli

MBS205577

0.05 mg

375 USD

Recombinant Protein

MMP2, 110-660aa, Human, His tag, E Coli

MMP2

Matrix metallopeptidase 2; CLG4; CLG4A; MMP-II; MONA; TBE-1

72 kDa type IV collagenase isoform 1 preproprotein; 72 kDa type IV collagenase; 72 kDa type IV collagenase; collagenase type IV-A; matrix metalloproteinase-2; matrix metalloproteinase-II; neutrophil gelatinase; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2; TBE-1PEX

MMP2

MMP2; MMP2; CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II; CLG4A; MMP-2

MMP2_HUMAN

660

MGSSHHHHHH SSGLVPRGSH MGSEFYNFFP RKPKWDKNQI TYRIIGYTPD LDPETVDDAF ARAFQVWSDV TPLRFSRIHD GEADIMINFG RWEHGDGYPF DGKDGLLAHA FAPGTGVGGD SHFDDDELWT LGEGQVVRVK YGNADGEYCK FPFLFNGKEY NSCTDTGRSD GFLWCSTTYN FEKDGKYGFC PHEALFTMGG NAEGQPCKFP FRFQGTSYDS CTTEGRTDGY RWCGTTEDYD RDKKYGFCPE TAMSTVGGNS EGAPCVFPFT FLGNKYESCT SAGRSDGKMW CATTANYDDD RKWGFCPDQG YSLFLVAAHE FGHAMGLEHS QDPGALMAPI YTYTKNFRLS QDDIKGIQEL YGASPDIDLG TGPTPTLGPV TPEICKQDIV FDGIAQIRGE IFFFKDRFIW RTVTPRDKPM GPLLVATFWP ELPEKIDAVY EAPQEEKAVF FAGNEYWIYS ASTLERGYPK PLTSLGLPPD VQRVDAAFNW SKNKKTYIFA GDKFWRYNEV KKKMDPGFPK LIADAWNAIP DNLDAVVDLQ GGGHSYFFKG AYYLKLENQS LKSVKFGSIK SDWLGC

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

0.25 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Metabolism

MMP2 protein of the matrix metalloproteinase (MMP) family is involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP2 is an enzyme which degrades type IV collagen, the major structural component of basement membranes. It plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Recombinant human MMP2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Kwan J.A., et al. (2004) FASEB J. 18:690-692

11342666

NP_004521

NM_004530.5

64.7 kDa (576aa)

AGE/RAGE Pathway (698754); ATF-2 Transcription Factor Network Pathway (138006); Activation Of Matrix Metalloproteinases Pathway (576264); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Axon Guidance Pathway (105688); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Developmental Biology Pathway (477129)

This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

MMP2: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly- -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Interacts (via the C-terminal hemopexin-like domains- containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B. Aspirin appears to inhibit expression. Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. Inhibited by histatin-3 1/24 (histatin-5). Belongs to the peptidase M10A family. Protein type: EC 3.4.24.24; Protease; Secreted, signal peptide; Cell development/differentiation; Motility/polarity/chemotaxis; Secreted; Apoptosis. Chromosomal Location of Human Ortholog: 16q12.2. Cellular Component: proteinaceous extracellular matrix; extracellular space; sarcomere; mitochondrion; extracellular region; plasma membrane; nucleus. Molecular Function: protein binding; zinc ion binding; serine-type endopeptidase activity; metalloendopeptidase activity. Biological Process: axon guidance; intramembranous ossification; extracellular matrix organization and biogenesis; proteolysis; blood vessel maturation; extracellular matrix disassembly; collagen catabolic process; cellular protein metabolic process; positive regulation of innate immune response; ephrin receptor signaling pathway; response to hypoxia; angiogenesis; embryo implantation. Disease: Multicentric Osteolysis, Nodulosis, And Arthropathy

0.05 mg

375 USD

0.25 mg

925