GLuD1, 54-558aa, Human, His tag, E Coli | MyBioSource MBS205535 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

GLuD1, 54-558aa, Human, His tag, E Coli

catalog :

MBS205535

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS205535

products type :

Recombinant Protein

products full name :

GLuD1, 54-558aa, Human, His tag, E Coli

products short name :

GLuD1

products name syn :

Glutamate dehydrogenase 1; mitochondrial; GDH; GDH1; GLuD

other names :

glutamate dehydrogenase 1, mitochondrial; Glutamate dehydrogenase 1, mitochondrial; glutamate dehydrogenase 1, mitochondrial; GDH 1; glutamate dehydrogenase (NAD(P)+); glutamate dehydrogenase 1

products gene name :

GLuD1

other gene names :

GLUD1; GLUD1; GDH; GDH1; GLUD; GLUD; GDH 1

uniprot entry name :

DHE3_HUMAN

sequence length :

558

sequence :

MGSSHHHHHH SSGLVPRGSH MGSSEAVADR EDDPNFFKMV EGFFDRGASI VEDKLVEDLR TRESEEQKRN RVRGILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTDNELEK ITRRFTMELA KKGFIGPGID VPAPDMSTGE REMSWIADTY ASTIGHYDIN AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGFG DKTFVVQGFG NVGLHSMRYL HRFGAKCIAV GESDGSIWNP DGIDPKELED FKLQHGSILG FPKAKPYEGS ILEADCDILI PAASEKQLTK SNAPRVKAKI IAEGANGPTT PEADKIFLER NIMVIPDLYL NAGGVTVSYF EWLKNLNHVS YGRLTFKYER DSNYHLLMSV QESLERKFGK HGGTIPIVPT AEFQDRISGA SEKDIVHSGL AYTMERSARQ IMRTAMKYNL GLDLRTAAYV NAIEKVFKVY NEAGVTFT

purity :

> 80% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

concentration :

1mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Metabolism

products description :

Glutamate dehydrogenase 1, mitochondrial precursor, also known as GLuD1, belongs to the Glu/Leu/Phe/Val dehydrogenases family. GLuD1 has a central role in nitrogen metabolism in plants and animals. GLuD1 is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate. Mutations in the gene encoding GLuD1 causes hyperinsulinism-hyperammonemia syndrome (HHS), which is an inherited condition characterized by high insulin and ammonia levels in the blood. GLuD1 may also be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. Recombinant human GLuD1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

products references :

Mastorodemos V., et al. (2009) Biochem Cell Biol. 87:505-516. Tanizawa Y., et al. (2002) Diabetes. 51:712-717.

ncbi gi num :

4885281

ncbi acc num :

NP_005262

ncbi gb acc num :

NM_005271.3

ncbi mol weight :

58.4 kDa (528aa)

ncbi pathways :

Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (106173); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); D-Glutamine And D-glutamate Metabolism Pathway (82971); D-Glutamine And D-glutamate Metabolism Pathway (340); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169)

ncbi summary :

This gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of this gene are present in humans.[provided by RefSeq, Sep 2009]

uniprot summary :

GLUD1: May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. Homohexamer. Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP. ADP can occupy the NADH binding site and activate the enzyme. Belongs to the Glu/Leu/Phe/Val dehydrogenases family. Protein type: EC 1.4.1.3; Mitochondrial; Amino Acid Metabolism - arginine and proline; Energy Metabolism - nitrogen; Oxidoreductase; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Amino Acid Metabolism - alanine, aspartate and glutamate. Chromosomal Location of Human Ortholog: 10q23.3. Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm. Molecular Function: identical protein binding; protein binding; glutamate dehydrogenase activity; GTP binding; glutamate dehydrogenase [NAD(P)+] activity; ADP binding; ATP binding. Biological Process: glutamate catabolic process; substantia nigra development; positive regulation of insulin secretion; glutamine metabolic process; amino acid biosynthetic process; glutamate biosynthetic process. Disease: Hyperinsulinemic Hypoglycemia, Familial, 6

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage