protein

RNASE3, 28-160aa, Human, His tag, E Coli

MBS205476

0.1 mg

310 USD

Recombinant Protein

RNASE3, 28-160aa, Human, His tag, E Coli

RNASE3

Eosinophil cationic protein; ECP; RNS3; Ribonuclease 3

Ribonuclease, RNase A family, 3 (eosinophil cationic protein); Eosinophil cationic protein; eosinophil cationic protein; RNase 3; cytotoxic ribonuclease; ribonuclease 3; ribonuclease, RNase A family, 3; Ribonuclease 3; RNase 3

RNASE3

RNASE3; RNASE3; ECP; RNS3; ECP; RNS3; ECP; RNase 3

ECP_HUMAN

160

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRP PQFTRAQWFA IQHISLNPPR CTIAMRAINN YRWRCKNQNT FLRTTFANVV NVCGNQSIRC PHNRTLNNCH RSRFRVPLLH CDLINPGAQN ISNCRYADRP GRRFYVVACD NRDPRDSPRY PVVPVHLDTT I

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Enzymes & Proteases

RNASE3 is cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. It possesses a wide variety of biological activities. This protein exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains and promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Recombinant human RNASE3 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Adu B, Dodoo D, Adukpo S, et al. (2011). PLoS One. 6(12):e29465. Torrent M, Nogues MV, Boix E, et al. (2011). J Mol Recognit. 24(1):90-100.

64652956

AAH96060

19.9 kDa (171aa)

Asthma Pathway (83120); Asthma Pathway (532)

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]

RNASE3: Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Belongs to the pancreatic ribonuclease family. Protein type: Secreted, signal peptide; Secreted; EC 3.1.27.-; Ribonuclease. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: extracellular space; extracellular region. Molecular Function: nucleic acid binding; endonuclease activity; ribonuclease activity. Biological Process: defense response to Gram-positive bacterium; innate immune response in mucosa; RNA catabolic process; antibacterial humoral response

0.1 mg

310 USD

0.5 mg

720