protein

SET, 1-290aa, Human, His tag, E Coli

MBS205385

0.1 mg

310 USD

Recombinant Protein

SET, 1-290aa, Human, His tag, E Coli

SET

Protein SET isoform 1; 2PP2A; I2PP2A; IGAAD; IPP2A2; PHAPII; TAF-I; TAF-IBETA

protein SET isoform 1; Protein SET; protein SET; HLA-DR-associated protein II; SET nuclear oncogene; SET translocation (myeloid leukemia-associated); Template-Activating Factor-I, chromatin remodelling factor; inhibitor of granzyme A-activated DNase; inhibitor-2 of protein phosphatase-2A; phosphatase 2A inhibitor I2PP2A; protein phosphatase type 2A inhibitor; SET nuclear proto-oncogene; HLA-DR-associated protein II; Inhibitor of granzyme A-activated DNase; IGAAD; PHAPII; Phosphatase 2A inhibitor I2PP2A; I-2PP2A; Template-activating factor I; TAF-I

SET

SET; SET; 2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA; IGAAD; I-2PP2A; TAF-I

SET_HUMAN

290

MGSSHHHHHH SSGLVPRGSH MGSMAPKRQS PLPPQKKKPR PPPALGPEET SASAGLPKKG EKEQQEAIEH IDEVQNEIDR LNEQASEEIL KVEQKYNKLR QPFFQKRSEL IAKIPNFWVT TFVNHPQVSA LLGEEDEEAL HYLTRVEVTE FEDIKSGYRI DFYFDENPYF ENKVLSKEFH LNESGDPSSK STEIKWKSGK DLTKRSSQTQ NKASRKRQHE EPESFFTWFT DHSDAGADEL GEVIKDDIWP NPLQYYLVPD MDDEEGEGEE DDDDDEEEEG LEDIDEEGDE DEGEEDEDDD EGEEGEEDEG EDD

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT.

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Signal Transduction

SET inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. Recombinant human SET protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Karetsou Z, Emmanouilidou A, et al. (2009). BMC Biochem. 10:10. Li M, Makkinje A, et al. (1996). J Biol Chem. 271(19):11059-62.

170763500

NP_001116293

NM_001122821.1

35.9 kDa (313aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)

Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Condensation Of Prophase Chromosomes Pathway (1127547); Gene Expression Pathway (105937); HuR Stabilizes MRNA Pathway (187216); M Phase Pathway (105812); Mitotic Prophase Pathway (105813); Regulation Of MRNA Stability By Proteins That Bind AU-rich Elements Pathway (187211)

The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

SET: a potent inhibitor of protein phosphatase 2A involved in apoptosis, transcription, nucleosome assembly and histone binding. The translocation t(6;9)(q21;q34.1) NUP214/CAN is found in some cases of acute undifferentiated leukemia (AUL). Two alternatively spliced isoforms have been described. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. Both isoforms inhibit the acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Protein type: Inhibitor; DNA-binding; DNA replication; Oncoprotein; Nuclear receptor co-regulator; Apoptosis. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: nucleoplasm; protein complex; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; cytosol; nucleus. Molecular Function: protein binding; DNA binding; histone binding; protein phosphatase type 2A regulator activity; protein phosphatase inhibitor activity. Biological Process: nucleosome assembly; nucleosome disassembly; regulation of catalytic activity; negative regulation of catalytic activity; nucleocytoplasmic transport; gene expression; mitotic cell cycle; negative regulation of neuron apoptosis; DNA replication; negative regulation of transcription, DNA-dependent; negative regulation of histone acetylation

0.1 mg

310 USD

0.5 mg

720