protein

RNASE7, 29-156aa, Human His tag, E Coli

MBS205343

0.05 mg

375 USD

Recombinant Protein

RNASE7, 29-156aa, Human His tag, E Coli

RNASE7

Ribonuclease; RNase A family; 7; MGC133220; Ribonuclease RNase A family 7; RNase 7; Ribonuclease 7; Skin-derived antimicrobial protein 2

ribonuclease, RNase A family, 7; Ribonuclease 7; ribonuclease 7; RNase 7; SAP-2; skin-derived antimicrobial protein 2; ribonuclease, RNase A family, 7; Skin-derived antimicrobial protein 2; SAP-2

RNASE7

RNASE7; RNASE7; RNase 7; SAP-2

RNAS7_HUMAN

156

MGSSHHHHHH SSGLVPRGSH MGSKPKGMTS SQWFKIQHMQ PSPQACNSAM KNINKHTKRC KDLNTFLHEP FSSVAATCQT PKIACKNGDK NCHQSHGPVS LTMCKLTSGK YPNCRYKEKR QNKSYVVACK PPQKKDSQQF HLVPVHLDRV L

> 90% by SDS - PAGE

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 1mM DTT

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Epigenomics

RNASE7 is one of the final RNase A superfamily ribonucleases. It was isolated from skin-derived stratum corneum. This protein exhibited potent ribonuclease activity and thus may contribute to the well known ribonuclease activity of human skin. It revealed broad spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity against a vancomycin-resistant Enterococcus faecium. Recombinant human RNASE7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Zhang J. et al. (2003) Nucleic Acids Res. 31:602-607. Harder J. et al. (2002) J Biol Chem. 277:46779-46784.

14211885

NP_115961.1

NM_032572.2

16.9 kDa(151aa) confirmed by MALDI-TOF

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein has broad-spectrum antimicrobial activity against bacteria and fungi. [provided by RefSeq, Oct 2014]

RNASE7: Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% 30 nM) against a vancomycin resistant Enterococcus faecium. Belongs to the pancreatic ribonuclease family. Protein type: Ribonuclease; Secreted; RNA-binding; Secreted, signal peptide; EC 3.1.27.-. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: extracellular region. Molecular Function: peptidoglycan binding; nucleic acid binding; lipopolysaccharide binding; endonuclease activity; ribonuclease activity. Biological Process: defense response to Gram-positive bacterium; response to bacterium; antibacterial humoral response; innate immune response; membrane disruption in another organism; defense response to Gram-negative bacterium

0.05 mg

375 USD

0.25 mg

925