catalog number :
MBS205290
products type :
Recombinant Protein
products full name :
MBP, 27-392aa, E Coli, His tag, E Coli
products short name :
[MBP]
products name syn :
[Cytoplasmic maltose-binding protein; DPH4; JJJ3; ZCSL3]
other names :
[maltose transporter subunit; Maltose-binding periplasmic protein; maltose transporter subunit; MBP; MMBP; Maltodextrin-binding protein]
products gene name :
[MBP]
other gene names :
[malE; malE; ECK4026; JW3994; malJ]
sequence :
MGSSHHHHHH SSGLVPRGSH MGSMKIEEGK LVIWINGDKG YNGLAEVGKK FEKDTGIKVT VEHPDKLEEK FPQVAATGDG PDIIFWAHDR FGGYAQSGLL AEITPDKAFQ DKLYPFTWDA VRYNGKLIAY PIAVEALSLI YNKDLLPNPP KTWEEIPALD KELKAKGKSA LMFNLQEPYF TWPLIAADGG YAFKYENGKY DIKDVGVDNA GAKAGLTFLV DLIKNKHMNA DTDYSIAEAA FNKGETAMTI NGPWAWSNID TSKVNYGVTV LPTFKGQPSK PFVGVLSAGI NAASPNKELA KEFLENYLLT DEGLEAVNKD KPLGAVALKS YEEELAKDPR IAATMENAQK GEIMPNIPQM SAFWYAVRTA VINAASGRQT VDEALKDAQT NSSSNNNNNN NNNNLGIEGR
purity :
> 95% by SDS-PAGE
form :
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
concentration :
0.5 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
image1 heading :
SDS-Page
other info1 :
Domain: 1-387aa. Tag: His-Tag
products categories :
Fusion Tag & Detection
products description :
Cytoplasmic maltose-binding protein, also known as MBP, is a protein related with the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has been used to increase the yield of its fusion partner in many cases. In addition, MBP is often able to promote the solubility of polypeptides to which it is fused. Recombinant E Coli MBP protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
products references :
Fox JD., et al. (2001) Protein Sci. 10 (3):622-30; Riggs P., et al. (2000) Mol Biotechnol. 15:51-63.
ncbi acc num :
NP_418458.1
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
44.9kDa (410aa), confirmed by MALDI-TOF
ncbi pathways :
ABC Transporters Pathway (1112); ABC Transporters Pathway (436); Bacterial Chemotaxis Pathway (1116); Bacterial Chemotaxis Pathway (438); Maltose/maltodextrin Transport System Pathway (405230); Maltose/maltodextrin Transport System Pathway (468371)
ncbi summary :
Biofilm microarray result was validated with RT-PCR. [More information is available at EcoGene: EG10554]. In addition to being the periplasmic substrate-binding component of the maltose ABC transporter, MalE with bound substrate is also able to bind to the chemoreceptor Tar to induce chemotaxis toward maltose. [More information is available at EcoCyc: EG10554].
uniprot summary :
Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.