protein

GOT2, 30-430aa Human, His tag, E Coli

MBS205234

0.1 mg

310 USD

Recombinant Protein

GOT2, 30-430aa Human, His tag, E Coli

GOT2

Aspartate aminotransferase 2; mitochondrial; KAT4; KATIV; mitAAT

aspartate aminotransferase, mitochondrial isoform 1; Aspartate aminotransferase, mitochondrial; aspartate aminotransferase, mitochondrial; FABP-1; FABPpm; aspartate aminotransferase 2; aspartate transaminase 2; fatty acid-binding protein; glutamate oxaloacetate transaminase 2; glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2); kynurenine aminotransferase 4; kynurenine aminotransferase IV; kynurenine--oxoglutarate transaminase 4; kynurenine--oxoglutarate transaminase IV; mAspAT; plasma membrane-associated fatty acid-binding protein; transaminase A; glutamic-oxaloacetic transaminase 2, mitochondrial; Fatty acid-binding protein; FABP-1; Glutamate oxaloacetate transaminase 2; Kynurenine aminotransferase 4; Kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; Plasma membrane-associated fatty acid-binding protein; FABPpm; Transaminase A

GOT2

GOT2; GOT2; KAT4; KATIV; mitAAT; mAspAT; FABP-1; FABPpm

AATM_HUMAN

430

MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Metabolism

GOT2, also known as glutamate oxaloacetate transaminase 2, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. glutamate oxaloacetate transaminas is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Guidetti P, Amori L, et al. (2007). J Neurochem. 102(1):103-11. Lain B, Iriarte A, et al. (1995). J Biol Chem. 270(42):24732-9.

73486658

NP_002071

NM_002080.3

47 kDa (424aa) confirmed by MALDI-TOF

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); 4-hydroxyproline Degradation Pathway (142337); Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (106173); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (790012)

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

GOT2: Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Protein type: Mitochondrial; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.7; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - tyrosine; Transferase; EC 2.6.1.1. Chromosomal Location of Human Ortholog: 16q21. Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane; plasma membrane. Molecular Function: kynurenine-oxoglutarate transaminase activity; aspartate transaminase activity; pyridoxal phosphate binding. Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; aspartate biosynthetic process; glucose metabolic process; pathogenesis; gluconeogenesis; 2-oxoglutarate metabolic process; response to ethanol; 4-hydroxyproline catabolic process; carbohydrate metabolic process; aspartate catabolic process; aspartate metabolic process; amino acid biosynthetic process; glutamate catabolic process to 2-oxoglutarate; fatty acid transport

0.1 mg

310 USD

0.5 mg

720