EED, 1-441aa, Human, His tag, E Coli | MyBioSource MBS205227 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

EED, 1-441aa, Human, His tag, E Coli

catalog :

MBS205227

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS205227

products type :

Recombinant Protein

products full name :

EED, 1-441aa, Human, His tag, E Coli

products short name :

EED

products name syn :

Polycomb protein EED isoform a; embryonic ectoderm development; HEED; WAIT1

other names :

polycomb protein EED isoform a; Polycomb protein EED; polycomb protein EED; WD protein associating with integrin cytoplasmic tails 1; embryonic ectoderm development; WD protein associating with integrin cytoplasmic tails 1; WAIT-1

products gene name :

EED

other gene names :

EED; EED; HEED; WAIT1; hEED; WAIT-1

uniprot entry name :

EED_HUMAN

sequence length :

441

sequence :

MGSSHHHHHH SSGLVPRGSH MGSMSEREVS TAPAGTDMPA AKKQKLSSDE NSNPDLSGDE NDDAVSIESG TNTERPDTPT NTPNAPGRKS WGKGKWKSKK CKYSFKCVNS LKEDHNQPLF GVQFNWHSKE GDPLVFATVG SNRVTLYECH SQGEIRLLQS YVDADADENF YTCAWTYDSN TSHPLLAVAG SRGIIRIINP ITMQCIKHYV GHGNAINELK FHPRDPNLLL SVSKDHALRL WNIQTDTLVA IFGGVEGHRD EVLSADYDLL GEKIMSCGMD HSLKLWRINS KRMMNAIKES YDYNPNKTNR PFISQKIHFP DFSTRDIHRN YVDCVRWLGD LILSKSCENA IVCWKPGKME DDIDKIKPSE SNVTILGRFD YSQCDIWYMR FSMDFWQKML ALGNQVGKLY VWDLEVEDPH KAKCTTLTHH KCGAAIRQTS FSRDSSILIA VCDDASIWRW DRLR

purity :

> 85% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Epigenomics

products description :

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta 7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Recombinant EED protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Rietzler M, Bittner M, Kolanus W, Schuster A, Holzmann B (Nov 1998). J Biol Chem 273 (42): 27459-66. Schumacher A, Lichtarge O, Schwartz S, Magnuson T (Jan 1999). Genomics 54 (1): 79-88

ncbi gi num :

24041020

ncbi acc num :

NP_003788

ncbi gb acc num :

NM_003797.3

ncbi mol weight :

52.6kDa (464aa)

ncbi pathways :

Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Epigenetic Regulation Of Gene Expression Pathway (1127671); Gene Expression Pathway (105937); Oxidative Stress Induced Senescence Pathway (905993); PRC2 Methylates Histones And DNA Pathway (1127672)

ncbi summary :

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

EED: a member of the superfamily of Polycomb group (PcG) methyltransferase proteins with WD-40 repeats. PcG complexes mediate chromatin modifications that contribute to transcriptional silencing during development and carcinogenesis. A part of the Polycomb-repressive complex 2 (PRC2) along with EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. Interacts with HDAC, HDAC2, histone H1 and YY1. PRC2 trimethylates histone H3 on K9 and K27, transcriptionally repressing target gene including HOXC8, HOXA9, MYT1 and CDKN2A. Appears to be overexpressed in breast and colon cancers. Inhibitors of PRC2-mediated gene repression are being investigated as a cancer therapy. EED and EZH2 are also implicated in the silencing of the inactive X chromosome. Three human isoforms produced by alternative splicing or initiation have been described, and additional isoforms may be produced by alternative initiation. Protein type: Methyltransferase. Chromosomal Location of Human Ortholog: 11q14.2-q22.3. Cellular Component: nucleoplasm; pronucleus; ESC/E(Z) complex; sex chromatin; cytoplasm; nucleolus; nucleus. Molecular Function: identical protein binding; histone methyltransferase activity; protein binding; chromatin binding. Biological Process: histone methylation; genetic imprinting; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; negative regulation of transcription, DNA-dependent; regulation of gene expression, epigenetic

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage