PCBP1, 1-163aa, Human, His tag, E Coli | MyBioSource MBS205177 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

PCBP1, 1-163aa, Human, His tag, E Coli

catalog :

MBS205177

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS205177

products type :

Recombinant Protein

products full name :

PCBP1, 1-163aa, Human, His tag, E Coli

products short name :

PCBP1

products name syn :

Poly(rC) binding protein 1; hnRNP-E1; hnRNP-X; HNRPE1; HNRPX

other names :

poly(rC)-binding protein 1; Poly(rC)-binding protein 1; poly(rC)-binding protein 1; poly(rC)-binding protein 1; alpha-CP1; epididymis secretory protein Li 85; heterogeneous nuclear ribonucleoprotein E1; heterogenous nuclear ribonucleoprotein E1; heterogenous nuclear ribonucleoprotein X; nucleic acid-binding protein SUB2.3; poly(rC) binding protein 1; Alpha-CP1; Heterogeneous nuclear ribonucleoprotein E1; hnRNP E1; Nucleic acid-binding protein SUB2.3

products gene name :

PCBP1

other gene names :

PCBP1; PCBP1; HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1; hnRNP E1

uniprot entry name :

PCBP1_HUMAN

sequence length :

356

sequence :

MGSSHHHHHH SSGLVPRGSH MGSMDAGVTE SGLNVTLTIR LLMHGKEVGS IIGKKGESVK RIREESGARI NISEGNCPER IITLTGPTNA IFKAFAMIID KLEEDINSSM TNSTAASRPP VTLRLVVPAT QCGSLIGKGG CKIKEIREST GAQVQVAGDM LPNSTERAIT IAGVPQSVTE CVKQIC

purity :

> 85% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Epigenomics

products description :

PCBP1 appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promotes poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP1 is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. Recombinant human PCBP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Beausoleil S.A., et al. (2006) Nat. Biotechnol. 24:1285-1292. Yu L.-R., et al. (2007) J. Proteome Res. 6:4150-4162.

ncbi gi num :

222352151

ncbi acc num :

NP_006187

ncbi gb acc num :

NM_006196.3

ncbi mol weight :

19.6 kDa (186aa) confirmed by MALDI-TOF

ncbi pathways :

Gene Expression Pathway (105937); Processing Of Capped Intron-Containing Pre-mRNA Pathway (160950); Spliceosome Pathway (125136); Spliceosome Pathway (124832); MRNA Splicing Pathway (105951); MRNA Splicing - Major Pathway (105952)

ncbi summary :

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]

uniprot summary :

hnRNP E1: Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands. Protein type: Translation; RNA splicing; RNA-binding. Chromosomal Location of Human Ortholog: 2p13-p12. Cellular Component: nucleoplasm; intracellular membrane-bound organelle; membrane; cytoplasm; ribonucleoprotein complex. Molecular Function: protein binding; RNA binding; single-stranded DNA binding. Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage