antibody

Cy3-Linked Monoclonal Antibody to Tryptase (TPS)

MBS2051186

0.1 mg

295 USD

monoclonal

mouse

Cy3

[C10]

western blot, immunohistochemistry, immunocytochemistry

Antibody

Cy3-Linked Monoclonal Antibody to Tryptase (TPS)

[Tryptase (TPS)]

[tryptase; Tryptase alpha/beta-1; tryptase alpha/beta-1; tryptase alpha/beta 1; Tryptase I; Tryptase alpha-1]

[TPS]

[TPSAB1; TPSAB1; TPS1; TPS2; TPSB1; TPS1; TPS2; TPSB1; Tryptase-1]

m

[C10]

Mouse

Protein A + Protein G affinity chromatography.

Supplied as solution form in PBS, pH7.4, 50% glycerol 0.05% Proclin-300.

0.62mg/ml

Storage: Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 24 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western blotting: 0.5-2ug/mL. Immunohistochemistry: 5-20ug/mL. Immunocytochemistry: 5-20ug/mL. Optimal working dilutions must be determined by end user.

Organism Species: Homo sapiens (Human). Source: Monoclonal antibody preparation. Label: Cy3. Traits: Liquid. Immunogen: Recombinant TPS (Gly161~Tyr262) expressed in E.coli.

Unconjugated Antibody: The unconjugated antibody version of this item is also available as catalog #MBS2085885

1182067

AAA86934.1

Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262)

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

TPSAB1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.21.59; Protease; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: extracellular matrix; extracellular region; extracellular space. Molecular Function: protein binding; serine-type endopeptidase activity; serine-type peptidase activity. Biological Process: defense response; extracellular matrix disassembly

0.1 mg

295 USD

0.2 mg

420

1 mg

995