SPN, 20-253aa, Human, His tag, E Coli | MyBioSource MBS205051 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

SPN, 20-253aa, Human, His tag, E Coli

catalog :

MBS205051

quantity :

0.1 mg

price :

375 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS205051

products type :

Recombinant Protein

products full name :

SPN, 20-253aa, Human, His tag, E Coli

products short name :

SPN

products name syn :

leukosialin precursor; CD43; GALGP; GPL115; LSN

other names :

leukosialin; Leukosialin; leukosialin; galactoglycoprotein; leukocyte sialoglycoprotein; sialophorin (gpL115, leukosialin, CD43); sialophorin; Galactoglycoprotein; GALGP; Leukocyte sialoglycoprotein; Sialophorin; CD_antigen: CD43

products gene name :

SPN

other gene names :

SPN; SPN; LSN; CD43; GALGP; GPL115; CD43; GALGP

uniprot entry name :

LEUK_HUMAN

sequence length :

400

sequence :

MGSSHHHHHH SSGLVPRGSH MGSSTTAVQT PTSGEPLVST SEPLSSKMYT TSITSDPKAD STGDQTSALP PSTSINEGSP LWTSIGASTG SPLPEPTTYQ EVSIKMSSVP QETPHATSHP AVPITANSLG SHTVTGGTIT TNSPETSSRT SGAPVTTAAS SLETSRGTSG PPLTMATVSL ETSKGTSGPP VTMATDSLET STGTTGPPVT MTTGSLEPSS GASGPQVSSV KLSTMMSPTT STNASTVPFR NPDENSR

purity :

> 85% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Stem Cell

products description :

SPN is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. Recombinant human SPN protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Park,H.J., et al. (2012) Am. J. Surg. Pathol. 36 (8), 1150-1157. Balikova,A., et al. (2012) Int. J. Oncol. 41 (1), 299-309

ncbi gi num :

4507181

ncbi acc num :

NP_003114

ncbi gb acc num :

NM_003123.4

ncbi mol weight :

25.8kDa (257aa) confirmed by MALDI-TOF. (Molecular size on SDS-PAGE will appear higher)

ncbi pathways :

Basigin Interactions Pathway (106065); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Hemostasis Pathway (106028)

ncbi summary :

The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011]

uniprot summary :

sialophorin: One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. Interacts with HIPK2 via the cytoplasmic domain. Interacts with RDX. Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. Protein type: Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: extracellular space; cell surface; membrane; integral to plasma membrane; plasma membrane; basement membrane; uropod; external side of plasma membrane. Molecular Function: protein binding; transmembrane receptor activity. Biological Process: positive regulation of tumor necrosis factor biosynthetic process; regulation of defense response to virus; establishment and/or maintenance of cell polarity; negative regulation of cell adhesion; chemotaxis; signal transduction; negative thymic T cell selection; negative regulation of T cell proliferation; cell surface receptor linked signal transduction; response to protozoan; T cell costimulation; defense response to bacterium; cellular defense response; immune response; positive regulation of T cell proliferation; positive regulation of protein amino acid phosphorylation; blood coagulation; negative regulation of type IV hypersensitivity; leukocyte migration

size1 :

0.1 mg

price1 :

375 USD

size2 :

0.5 mg

price2 :

925

more info or order :

MyBioSource product webpage