protein

TRIM28, 366-802aa, Human, His tag, E Coli

MBS204925

0.1 mg

310 USD

Recombinant Protein

TRIM28, 366-802aa, Human, His tag, E Coli

TRIM28

Transcription intermediary factor 1-beta; Tripartite motif containing 28; KAP1; RNF96; TF1B; TIF1B

transcription intermediary factor 1-beta; Transcription intermediary factor 1-beta; transcription intermediary factor 1-beta; E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; RING finger protein 96; TIF1-beta; nuclear corepressor KAP-1; protein phosphatase 1, regulatory subunit 157; transcriptional intermediary factor 1-beta; tripartite motif-containing 28; tripartite motif-containing protein 28; tripartite motif containing 28; E3 SUMO-protein ligase TRIM28 (EC:6.3.2.-); KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; RING finger protein 96; Tripartite motif-containing protein 28

TRIM28

TRIM28; TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157; KAP1; RNF96; TIF1B; TIF1-beta; KAP-1; KRIP-1

TIF1B_HUMAN

835

MGSSHHHHHH SSGLVPRGSH MGSKLIYFQL HRALKMIVDP VEPHGEMKFQ WDLNAWTKSA EAFGKIVAER PGTNSTGPAP MAPPRAPGPL SKQGSGSSQP MEVQEGYGFG SGDDPYSSAE PHVSGVKRSR SGEGEVSGLM RKVPRVSLER LDLDLTADSQ PPVFKVFPGS TTEDYNLIVI ERGAAAAATG QPGTAPAGTP GAPPLAGMAI VKEEETEAAI GAPPTATEGP ETKPVLMALA EGPGAEGPRL ASPSGSTSSG LEVVAPEGTS APGGGPGTLD DSATICRVCQ KPGDLVMCNQ CEFCFHLDCH LPALQDVPGE EWSCSLCHVL PDLKEEDGSL SLDGADSTGV VAKLSPANQR KCERVLLALF CHEPCRPLHQ LATDSTFSLD QPGGTLDLTL IRARLQEKLS PPYSSPQEFA QDVGRMFKQF NKLTEDKADV QSIIGLQRFF ETRMNEAFGD

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Epigenomics

TRIM28 mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Recombinant human TRIM28 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Friedman J.R,. et al. (1996). Genes Dev. 10:2067-2078

5032179

NP_005753

NM_005762.2

48.7 kDa (460aa)

C-MYB Transcription Factor Network Pathway (138073); Corticotropin-releasing Hormone Pathway (920957); E2F Transcription Factor Network Pathway (137934); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); P53 Pathway (138067)

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

TIF1B: an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Protein type: Protein kinase, atypical; Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.-; Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Kinase, protein; ATYPICAL group; TIF1 family. Chromosomal Location of Human Ortholog: 19q13.4. Cellular Component: nucleoplasm; nuclear heterochromatin; nucleus. Molecular Function: protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; transcription corepressor activity; transcription factor activity; ligase activity; protein kinase activity. Biological Process: transcription initiation from RNA polymerase II promoter; convergent extension involved in axis elongation; positive regulation of DNA repair; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein oligomerization; protein sumoylation; positive regulation of transcription factor import into nucleus; innate immune response; epithelial to mesenchymal transition; gene expression; DNA methylation during embryonic development; negative regulation of transcription, DNA-dependent; embryo implantation

0.1 mg

310 USD

0.5 mg

720