TuBB3, 1-450aa, Human, His tag, E Coli | MyBioSource MBS204798 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

TuBB3, 1-450aa, Human, His tag, E Coli

catalog :

MBS204798

quantity :

0.05 mg

price :

375 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204798

products type :

Recombinant Protein

products full name :

TuBB3, 1-450aa, Human, His tag, E Coli

products short name :

TuBB3

products name syn :

Tubulin; beta 3 class III; beta-4; CDCBM; CFEOM3A; TuBB4

other names :

tubulin beta-3 chain isoform 1; Tubulin beta-3 chain; tubulin beta-3 chain; class III beta-tubulin; tubulin beta-4 chain; tubulin beta-III; tubulin, beta 3 class III; Tubulin beta-4 chain; Tubulin beta-III

products gene name :

TuBB3

other gene names :

TUBB3; TUBB3; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A; TUBB4

uniprot entry name :

TBB3_HUMAN

sequence length :

450

sequence :

MGSSHHHHHH SSGLVPRGSH MGSMREIVHI QAGQCGNQIG AKFWEVISDE HGIDPSGNYV GDSDLQLERI SVYYNEASSH KYVPRAILVD LEPGTMDSVR SGAFGHLFRP DNFIFGQSGA GNNWAKGHYT EGAELVDSVL DVVRKECENC DCLQGFQLTH SLGGGTGSGM GTLLISKVRE EYPDRIMNTF SVVPSPKVSD TVVEPYNATL SIHQLVENTD ETYCIDNEAL YDICFRTLKL ATPTYGDLNH LVSATMSGVT TSLRFPGQLN ADLRKLAVNM VPFPRLHFFM PGFAPLTARG SQQYRALTVP ELTQQMFDAK NMMAACDPRH GRYLTVATVF RGRMSMKEVD EQMLAIQSKN SSYFVEWIPN NVKVAVCDIP PRGLKMSSTF IGNSTAIQEL FKRISEQFTA MFRRKAFLHW YTGEGMDEME FTEAESNMND LVSEYQQYQD ATAEEEGEMY EDDEEESEAQ GPK

purity :

> 90% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl,10% glycerol, 1mM DTT

concentration :

0.25 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Cytoskeleton

products description :

Tubulin, beta 3 class III, also known as TuBB3, is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TuBB3 plays a critical role in proper axon guidance and mantainance. Recombinant human TuBB3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Katsetos C.D., et al. (2003) J. Child Neurol. 18:851-866. Tischfield M.A., et al. (2010) Cell. 140:74-87.

ncbi gi num :

50592996

ncbi acc num :

NP_006077

ncbi gb acc num :

NM_006086.3

ncbi mol weight :

52.8 kDa (473aa)

ncbi pathways :

Chaperonin-mediated Protein Folding Pathway (106242); Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway (106243); Diurnally Regulated Genes With Circadian Orthologs Pathway (198813); Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway (106245); Gap Junction Pathway (83072); Gap Junction Pathway (483); Metabolism Of Proteins Pathway (106230); Orphan Transporters Pathway (1127546); Parkin-Ubiquitin Proteasomal System Pathway (700638); Pathogenic Escherichia Coli Infection Pathway (83103)

ncbi summary :

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

uniprot summary :

TUBB3: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Dimer of alpha and beta chains. Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. Belongs to the tubulin family. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 16q24.3. Cellular Component: microtubule; cell soma; axon; cytoplasm; dendrite; nucleus. Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; peptide binding. Biological Process: protein polymerization; mitosis; de novo posttranslational protein folding; axon guidance; cellular protein metabolic process; protein folding; transmembrane transport; microtubule-based process. Disease: Fibrosis Of Extraocular Muscles, Congenital, 3a, With Or Without Extraocular Involvement; Cortical Dysplasia, Complex, With Other Brain Malformations 1

size1 :

0.05 mg

price1 :

375 USD

size2 :

0.25 mg

price2 :

925

more info or order :

MyBioSource product webpage