DHODH, 31-395aa, Human, His tag, E Coli | MyBioSource MBS204782 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

DHODH, 31-395aa, Human, His tag, E Coli

catalog :

MBS204782

quantity :

0.01 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204782

products type :

Recombinant Protein

products full name :

DHODH, 31-395aa, Human, His tag, E Coli

products short name :

DHODH

products name syn :

Dihydroorotate dehydrogenase (quinone); mitochondrial; DHOdehase; POADS; uRA1

other names :

dihydroorotate dehydrogenase (quinone), mitochondrial; Dihydroorotate dehydrogenase (quinone), mitochondrial; dihydroorotate dehydrogenase (quinone), mitochondrial; dihydroorotate dehydrogenase (quinone), mitochondrial; dihydroorotate oxidase; human complement of yeast URA1; dihydroorotate dehydrogenase (quinone); Dihydroorotate oxidase

products gene name :

DHODH

other gene names :

DHODH; DHODH; URA1; POADS; DHOdehase; DHOdehase

uniprot entry name :

PYRD_HUMAN

sequence length :

395

sequence :

MGSSHHHHHH SSGLVPRGSH MGSHMTGDER FYAEHLMPTL QGLLDPESAH RLAVRFTSLG LLPRARFQDS DMLEVRVLGH KFRNPVGIAA GFDKHGEAVD GLYKMGFGFV EIGSVTPKPQ EGNPRPRVFR LPEDQAVINR YGFNSHGLSV VEHRLRARQQ KQAKLTEDGL PLGVNLGKNK TSVDAAEDYA EGVRVLGPLA DYLVVNVSSP NTAGLRSLQG KAELRRLLTK VLQERDGLRR VHRPAVLVKI APDLTSQDKE DIASVVKELG IDGLIVTNTT VSRPAGLQGA LRSETGGLSG KPLRDLSTQT IREMYALTQG RVPIIGVGGV SSGQDALEKI RAGASLVQLY TALTFWGPPV VGKVKRELEA LLKEQGFGGV TDAIGADHRR

purity :

> 90% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Metabolism

products description :

DHODH, also known as dihydroorotate dehydrogenase (quinone), mitochondrial, belongs to the dihydroorotate dehydrogenase family. DHODH catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. Recombinant human DHODH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Rawls J., et al. (2000) Eur. J. Biochem. 267:2079-2087. Liu S., et al. (2000) Structure. 8:25-33

ncbi gi num :

45006951

ncbi acc num :

NP_001352

ncbi gb acc num :

NM_001361.4

ncbi mol weight :

42.3 kDa (390aa) confirmed by MALDI-TOF

ncbi pathways :

Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Pyrimidine Biosynthesis Pathway (161056); Pyrimidine Metabolism Pathway (106281); Pyrimidine Metabolism Pathway (82946); Pyrimidine Metabolism Pathway (309); Uridine Monophosphate Biosynthesis, Glutamine (+ PRPP) = UMP Pathway (524498); Uridine Monophosphate Biosynthesis, Glutamine (+ PRPP) = UMP Pathway (468244)

ncbi summary :

The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]

uniprot summary :

DHODH: Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS); also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases. Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily. Protein type: Nucleotide Metabolism - pyrimidine; EC 1.3.5.2; Mitochondrial; Oxidoreductase; Membrane protein, integral. Chromosomal Location of Human Ortholog: 16q22. Cellular Component: nucleoplasm; mitochondrion; cell soma; mitochondrial inner membrane; integral to membrane; intrinsic to mitochondrial inner membrane. Molecular Function: ubiquinone binding; FMN binding; dihydroorotate dehydrogenase activity; drug binding. Biological Process: lactation; response to starvation; response to drug; de novo pyrimidine base biosynthetic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; positive regulation of apoptosis; pyrimidine nucleoside biosynthetic process; female pregnancy; response to caffeine. Disease: Postaxial Acrofacial Dysostosis

size1 :

0.01 mg

price1 :

310 USD

size2 :

0.05 mg

price2 :

720

more info or order :

MyBioSource product webpage