protein

uBA5, 1-404aa, human, His tag, E Coli

MBS204777

0.05 mg

375 USD

Recombinant Protein

uBA5, 1-404aa, human, His tag, E Coli

uBA5

ubiquitin-like modifier-activating enzyme 5; THIFP1; uBE1DC1

ubiquitin-like modifier-activating enzyme 5 isoform 1; Ubiquitin-like modifier-activating enzyme 5; ubiquitin-like modifier-activating enzyme 5; UBA5, ubiquitin-activating enzyme E1 homolog; UFM1-activating enzyme; ubiquitin-activating enzyme 5; ubiquitin-activating enzyme E1 domain-containing protein 1; ubiquitin-activating enzyme E1-domain containing 1; ubiquitin-like modifier activating enzyme 5; ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1

uBA5

UBA5; UBA5; THIFP1; UBE1DC1; UBE1DC1; Ubiquitin-activating enzyme 5

UBA5_HUMAN

404

MGSSHHHHHH SSGLVPRGSH MGSHMAESVE RLQQRVQELE RELAQERSLQ VPRSGDGGGG RVRIEKMSSE VVDSNPYSRL MALKRMGIVS DYEKIRTFAV AIVGVGGVGS VTAEMLTRCG IGKLLLFDYD KVELANMNRL FFQPHQAGLS KVQAAEHTLR NINPDVLFEV HNYNITTVEN FQHFMDRISN GGLEEGKPVD LVLSCVDNFE ARMTINTACN ELGQTWMESG VSENAVSGHI QLIIPGESAC FACAPPLVVA ANIDEKTLKR EGVCAASLPT TMGVVAGILV QNVLKFLLNF GTVSFYLGYN AMQDFFPTMS MKPNPQCDDR NCRKQQEEYK KKVAALPKQE VIQEEEEIIH EDNEWGIELV SEVSEEELKN FSGPVPDLPE GITVAYTIPK KQEDSVTELT VEDSGESLED LMAKMKNM

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl

0.5mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

ubiquitination

uBA5 (ubiquitin-like modifier activating enzyme 5) belongs to the ubiquitin-activating E1 family and uBA5 subfamily. ubiquitin and ubiquitin-like proteins are known to be covalently conjugated to a variety of cellular substrates via a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) plays a key role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. uBA5 had been proved to activate an ubiquitin-like protein, ubiquitin-fold modifier 1 (ufm1), by forming a high-energy thioester bond. It localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SuMO2. Recombinant human uBA5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Komatsu M., et al. (2004) EMBO J. 23:1977-1986. Zheng M., et al. (2008) J. Cell. Biochem. 104:2324-2334

13376212

NP_079094

NM_024818.3

47.4kDa (428aa) confirmed by MALDI-TOF

Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Immune System Pathway (106386)

This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also been identified. [provided by RefSeq, Jul 2008]

UBE1DC1: E1-like enzyme which activates UFM1 and SUMO2. Belongs to the ubiquitin-activating E1 family. UBA5 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ubiquitin conjugating system; Autophagy. Chromosomal Location of Human Ortholog: 3q22.1. Cellular Component: intracellular membrane-bound organelle; cytoplasm; cytosol; nucleus. Molecular Function: protein binding; metal ion binding; ATP binding. Biological Process: regulation of estrogen receptor signaling pathway

0.05 mg

375 USD

0.25 mg

925