protein

ATG4B, 1-393aa, Human, His tag, E Coli

MBS204719

0.1 mg

375 USD

Recombinant Protein

ATG4B, 1-393aa, Human, His tag, E Coli

ATG4B

Cysteine protease ATG4B; AuTL1; Autophagin-1

ATG4 autophagy related 4 homolog B (S. cerevisiae); Cysteine protease ATG4B; cysteine protease ATG4B; APG4 autophagy 4 homolog B; ATG4 autophagy related 4 homolog B; AUT-like 1 cysteine endopeptidase; autophagin-1; autophagy-related cysteine endopeptidase 1; autophagy-related protein 4 homolog B; hAPG4B; autophagy related 4B, cysteine peptidase; AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; hAPG4B

ATG4B

ATG4B; ATG4B; APG4B; AUTL1; APG4B; AUTL1; KIAA0943; hAPG4B

ATG4B_HUMAN

393

MDAATLTYDT LRFAEFEDFP ETSEPVWILG RKYSIFTEKD EILSDVASRL WFTYRKNFPA IGGTGPTSDT GWGCMLRCGQ MIFAQALVCR HLGRDWRWTQ RKRQPDSYFS VLNAFIDRKD SYYSIHQIAQ MGVGEGKSIG QWYGPNTVAQ VLKKLAVFDT WSSLAVHIAM DNTVVMEEIR RLCRTSVPCA GATAFPADSD RHCNGFPAGA EVTNRPSPWR PLVLLIPLRL GLTDINEAYV ETLKHCFMMP QSLGVIGGKP NSAHYFIGYV GEELIYLDPH TTQPAVEPTD GCFIPDESFH CQHPPCRMSI AELDPSIAVG FFCKTEDDFN DWCQQVKKLS LLGGALPMFE LVEQQPSHLA CPDVLNLSLD SSDVERLERF FDSEDEDFEI LSLLEHHHHH H

> 90% by SDS - PAGE

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT, 0.1mM PMSF

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

ubiquitination

ATG4B, also known as cysteine protease ATG4B, is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Recombinant human ATG4B protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.

Sugawara K., et al. (2005) J. Biol. Chem. 280:40058-40065. Kumanomidou T., et al. (2006) J. Mol. Biol. 355:612-618.

12653857

AAH00719

45.4 kDa (401aa) confirmed by MALDI-TOF

Regulation Of Autophagy Pathway 83058!!Regulation Of Autophagy Pathway 468

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

0.1 mg

375 USD