CHMP1B, 1-199aa, Human, His tag, E Coli | MyBioSource MBS204693 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

CHMP1B, 1-199aa, Human, His tag, E Coli

catalog :

MBS204693

quantity :

0.02 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204693

products type :

Recombinant Protein

products full name :

CHMP1B, 1-199aa, Human, His tag, E Coli

products short name :

CHMP1B

products name syn :

Charged multivesicular body protein 1b; C10orf2; C18-ORF2; C18orf2; CHMP1.5; hVps46-2; Vps46-2; Vps46B

other names :

charged multivesicular body protein 1b; Charged multivesicular body protein 1b; charged multivesicular body protein 1b; chromatin modifying protein 1B; chromatin-modifying protein 1b; vacuolar protein sorting 46-2; vacuolar protein sorting-associated protein 46-2; charged multivesicular body protein 1B; CHMP1.5; Chromatin-modifying protein 1b; CHMP1b; Vacuolar protein sorting-associated protein 46-2; Vps46-2; hVps46-2

products gene name :

CHMP1B

other gene names :

CHMP1B; CHMP1B; Vps46B; C10orf2; C18orf2; CHMP1.5; Vps46-2; C18-ORF2; hVps46-2; C18orf2; CHMP1b; Vps46-2; hVps46-2

uniprot entry name :

CHM1B_HUMAN

sequence length :

199

sequence :

MGSSHHHHHH SSGLVPRGSH MGSHMSNMEK HLFNLKFAAK ELSRSAKKCD KEEKAEKAKI KKAIQKGNME VARIHAENAI RQKNQAVNFL RMSARVDAVA ARVQTAVTMG KVTKSMAGVV KSMDATLKTM NLEKISALMD KFEHQFETLD VQTQQMEDTM SSTTTLTTPQ NQVDMLLQEM ADEAGLDLNM ELPQGQTGSV GTSVASAEQD ELSQRLARLR DQV

purity :

> 85% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

concentration :

0.25mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Cell cycle

products description :

CHMP1B, also known as charged multivesicular body protein 1b, is probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Recombinant human CHMP1B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Martin-Serrano J., et al. (2003) Proc. Natl. Acad. Sci. u.S.A. 100:12414-12419. Bajorek M., et al. (2009) Mol. Biol. Cell. 20:1360-1373

ncbi gi num :

31542306

ncbi acc num :

NP_065145

ncbi gb acc num :

NM_020412.4

ncbi mol weight :

24.7 kDa (223aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)

ncbi pathways :

ESCRT-III Complex Pathway (413475); ESCRT-III Complex Pathway (890627); Endocytosis Pathway (102279); Endocytosis Pathway (102181)

ncbi summary :

CHMP1B belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008]

uniprot summary :

CHMP1B: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT- III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B and SPAST to the midbody of dividing cells. Involved in HIV-1 p6- and p9-dependent virus release. Belongs to the SNF7 family. Chromosomal Location of Human Ortholog: 18p11.21. Cellular Component: membrane coat; late endosome membrane; cytosol. Molecular Function: protein domain specific binding; protein binding. Biological Process: establishment of protein localization; protein transport; cell separation during cytokinesis; vacuolar transport; cytokinesis; mitotic metaphase plate congression; nuclear organization and biogenesis

size1 :

0.02 mg

price1 :

310 USD

size2 :

0.1 mg

price2 :

720

more info or order :

MyBioSource product webpage