CBX3, 1-183aa, Human, His tag, E Coli | MyBioSource MBS204674 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

CBX3, 1-183aa, Human, His tag, E Coli

catalog :

MBS204674

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204674

products type :

Recombinant Protein

products full name :

CBX3, 1-183aa, Human, His tag, E Coli

products short name :

CBX3

products name syn :

Chromobox protein homolog 3; HECH; HP1-GAMMA; HP1Hs-gamma

other names :

chromobox protein homolog 3; Chromobox protein homolog 3; chromobox protein homolog 3; HP1 gamma homolog; chromobox homolog 3 (HP1 gamma homolog, Drosophila); heterochromatin protein 1 homolog gamma; heterochromatin protein HP1 gamma; heterochromatin-like protein 1; modifier 2 protein; chromobox homolog 3; HECH; Heterochromatin protein 1 homolog gamma; HP1 gamma; Modifier 2 protein

products gene name :

CBX3

other gene names :

CBX3; CBX3; HECH; HP1-GAMMA; HP1Hs-gamma; HP1 gamma

uniprot entry name :

CBX3_HUMAN

sequence length :

183

sequence :

MGSSHHHHHH SSGLVPRGSH MGSHMASNKT TLQKMGKKQN GKSKKVEEAE PEEFVVEKVL DRRVVNGKVE YFLKWKGFTD ADNTWEPEEN LDCPELIEAF LNSQKAGKEK DGTKRKSLSD SESDDSKSKK KRDAADKPRG FARGLDPERI IGATDSSGEL MFLMKWKDSD EADLVLAKEA NMKCPQIVIA FYEERLTWHS CPEDEAQ

purity :

> 90% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Epigenomics

products description :

CBX3, also known as chromobox protein homolog 3, seems to be involved in transcriptional silencing in heterochromatin-like complexes. This protein recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. It involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Recombinant human CBX3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Koike N., et al. (2000) FEBS Lett. 467:17-21. Lachner M., et al. (2001) Nature. 410:116-120.

ncbi gi num :

20544151

ncbi acc num :

NP_057671

ncbi gb acc num :

NM_016587.3

ncbi mol weight :

23.4 kDa (207aa), confirmed by MALDI-TOF

ncbi pathways :

Diurnally Regulated Genes With Circadian Orthologs Pathway (198813); Gene Expression Pathway (105937); RNA Polymerase I Chain Elongation Pathway (106555); RNA Polymerase I Transcription Pathway (106550); RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway (160948)

ncbi summary :

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.[provided by RefSeq, Mar 2011]

uniprot summary :

CBX3: Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at Lys-9 , leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Binds directly to CHAF1A. Interacts with histone H3 methylated at Lys-9 . Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2 and SP100. Interacts with TIF1A. Interacts with MIS12 and DSN1. Can interact directly with CBX5 via the chromoshadow domain. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 7p15.2. Cellular Component: nucleoplasm; nuclear inner membrane; spindle; nuclear envelope; nuclear heterochromatin; nucleus; chromatin. Molecular Function: identical protein binding; protein domain specific binding; protein binding; enzyme binding. Biological Process: chromatin remodeling; transcription, DNA-dependent; rhythmic process; negative regulation of transcription, DNA-dependent

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage