protein

FKBP1B, 1-108aa, Human, His tag, E Coli

MBS204669

0.1 mg

310 USD

Recombinant Protein

FKBP1B, 1-108aa, Human, His tag, E Coli

FKBP1B

Peptidyl-prolyl cis-trans isomerase FKBP1B; FKBP12.6; FKBP1L; OTK4; PKBP1L; PPIase

peptidyl-prolyl cis-trans isomerase FKBP1B isoform a; Peptidyl-prolyl cis-trans isomerase FKBP1B; peptidyl-prolyl cis-trans isomerase FKBP1B; 12.6 kDa FK506-binding protein; 12.6 kDa FKBP; FK506-binding protein 12.6; FK506-binding protein 1B; FKBP-12.6; FKBP-1B; PPIase FKBP1B; calstabin 2; h-FKBP-12; immunophilin FKBP12.6; rotamase; FK506 binding protein 1B, 12.6 kDa; 12.6 kDa FK506-binding protein; 12.6 kDa FKBP; FKBP-12.6; FK506-binding protein 1B; FKBP-1B; Immunophilin FKBP12.6; Rotamase; h-FKBP-12

FKBP1B

FKBP1B; FKBP1B; OTK4; FKBP1L; PKBP1L; PPIase; FKBP12.6; FKBP12.6; FKBP1L; FKBP9; OTK4; PPIase FKBP1B; 12.6 kDa FKBP; FKBP-12.6; FKBP-1B

FKB1B_HUMAN

108

MGSSHHHHHH SSGLVPRGSH RSMGVEIETI SPGDGRTFPK KGQTCVVHYT GMLQNGKKFD SSRDRNKPFK FRIGKQEVIK GFEEGAAQMS LGQRAKLTCT PDVAYGATGH PGVIPPNATL IFDVELLNLE

> 90% by SDS - PAGE

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT

0.25 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. 2. Add 10ul of recombinant FKBP1B protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM). 5. Record the increase in A405 nm for 30 minutes at 25C.

Heat Shock Proteins

FKBP1B, also known as peptidyl-prolyl cis-trans isomerase FKBP1B, is a member of the immunophilin protein family which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 (tacrolimus) and rapamycin (sirolimus). It is highly similar to the FK506-binding protein 1A. Its physiological role is thought to be in excitation-contraction coupling in cardiac muscle. Recombinant human FKBP1B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Arakawa H., et al. (1994) Biochem. Biophys. Res. 200:836-843. Deivanayagam C.C., et al. (2000) Acta Crystallogr. D 56:266-271

4758380

NP_004107

NM_004116.3

14.2 kDa (130aa), confirmed by MALDI-TOF

Integrated Pancreatic Cancer Pathway 711360!!Ion Channel Transport Pathway 187193!!Stimuli-sensing Channels Pathway 771568!!Transmembrane Transport Of Small Molecules Pathway 106572

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It is highly similar to the FK506-binding protein 1A. Its physiological role is thought to be in excitation-contraction coupling in cardiac muscle. There are two alternatively spliced transcript variants of this gene encoding different isoforms. [provided by RefSeq, Jul 2008]

0.1 mg

310 USD