MCL1, 1-327aa, Human, His tag, E Coli | MyBioSource MBS204656 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

MCL1, 1-327aa, Human, His tag, E Coli

catalog :

MBS204656

quantity :

0.05 mg

price :

375 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204656

products type :

Recombinant Protein

products full name :

MCL1, 1-327aa, Human, His tag, E Coli

products short name :

MCL1

products name syn :

induced myeloid leukemia cell differentiation protein Mcl-1; BCL2L3; mcl1/EAT; myeloid cell leukemia sequence 1; Bcl-2-like protein 3

other names :

induced myeloid leukemia cell differentiation protein Mcl-1 isoform 1; Induced myeloid leukemia cell differentiation protein Mcl-1; induced myeloid leukemia cell differentiation protein Mcl-1; bcl-2-like protein 3; bcl-2-related protein EAT/mcl1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related); myeloid cell leukemia 1; Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT

products gene name :

MCL1

other gene names :

MCL1; MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT; BCL2L3; Bcl2-L-3

uniprot entry name :

MCL1_HUMAN

sequence length :

350

sequence :

MGSSHHHHHH SSGLVPRGSH MFGLKRNAVI GLNLYCGGAG LGAGSGGATR PGGRLLATEK EASARREIGG GEAGAVIGGS AGASPPSTLT PDSRRVARPP PIGAEVPDVT ATPARLLFFA PTRRAAPLEE MEAPAADAIM SPEEELDGYE PEPLGKRPAV LPLLELVGES GNNTSTDGSL PSTPPPAEEE EDELYRQSLE IISRYLREQA TGAKDTKPMG RSGATSRKAL ETLRRVGDGV QRNHETAFQG MLRKLDIKNE DDVKSLSRVM IHVFSDGVTN WGRIVTLISF GAFVAKHLKT INQESCIEPL AESITDVLVR TKRDWLVKQR GWDGFVEFFH VEDLEGG

purity :

> 90% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT

concentration :

0.25 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Metabolism

products description :

MCL1, also known as BCL2L3, belongs to the Bcl-2 family. MCL1 is involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing. Recombinant human MCL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

products references :

Bingle C.D., et al. (2000) J. Biol. Chem. 275:22136-22146. Bae J., et al. (2000) J. Biol. Chem. 275:25255-25261

ncbi gi num :

11386165

ncbi acc num :

NP_068779

ncbi gb acc num :

NM_021960.4

ncbi mol weight :

37.2 kDa (347aa), confirmed by MALDI-TOF

ncbi pathways :

Apoptosis Pathway (198797); Apoptosis Modulation And Signaling Pathway (198822); Direct P53 Effectors Pathway (137939); E2F Transcription Factor Network Pathway (137934); HIF-1-alpha Transcription Factor Network Pathway (138045); IL-7 Signaling Pathway (198857); IL6-mediated Signaling Events Pathway (137932); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); PI3K-Akt Signaling Pathway (692234)

ncbi summary :

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

uniprot summary :

MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing. Protein type: Membrane protein, integral; Channel, misc.; Mitochondrial; Inhibitor; Apoptosis. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; cytosol; nucleus. Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding. Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; multicellular organismal development; response to cytokine stimulus; cell fate determination; cellular homeostasis

size1 :

0.05 mg

price1 :

375 USD

size2 :

0.25 mg

price2 :

925

more info or order :

MyBioSource product webpage