protein

ADH5, 1-374aa, Human, His tag, E Coli

MBS204610

0.1 mg

310 USD

Recombinant Protein

ADH5, 1-374aa, Human, His tag, E Coli

ADH5

Alcohol dehydrogenase class-3; ADHX; FDH

alcohol dehydrogenase class-3; Alcohol dehydrogenase class-3; alcohol dehydrogenase class-3; S-(hydroxymethyl)glutathione dehydrogenase; alcohol dehydrogenase (class III), chi polypeptide; alcohol dehydrogenase class chi chain; alcohol dehydrogenase class-III; formaldehyde dehydrogenase; glutathione-dependent formaldehyde dehydrogenase; alcohol dehydrogenase 5 (class III), chi polypeptide; Alcohol dehydrogenase 5; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-); FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.284)

ADH5

ADH5; ADH5; FDH; ADHX; ADH-3; FALDH; GSNOR; GSH-FDH; FALDH; FDH; GSH-FDH

ADHX_HUMAN

374

MGSSHHHHHH SSGLVPRGSH MGSHMANEVI KCKAAVAWEA GKPLSIEEIE VAPPKAHEVR IKIIATAVCH TDAYTLSGAD PEGCFPVILG HEGAGIVESV GEGVTKLKAG DTVIPLYIPQ CGECKFCLNP KTNLCQKIRV TQGKGLMPDG TSRFTCKGKT ILHYMGTSTF SEYTVVADIS VAKIDPLAPL DKVCLLGCGI STGYGAAVNT AKLEPGSVCA VFGLGGVGLA VIMGCKVAGA SRIIGVDINK DKFARAKEFG ATECINPQDF SKPIQEVLIE MTDGGVDYSF ECIGNVKVMR AALEACHKGW GVSVVVGVAA SGEEIATRPF QLVTGRTWKG TAFGGWKSVE SVPKLVSEYM SKKIKVDEFV THNLSFDEIN KAFELMHSGK SIRTVVKI

> 90% by SDS - PAGE

Liquid. 20mM Tris-HCl buffer (pH7.5) containing 20% glycerol 0.1M Nacl, 1mM DTT

1.0 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Signal Transduction

ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. Recombinant human ADH5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Zhang,X. et al. (2010) Carcinogenesis 31 (12), 2118-2123. Shimada,M. et al. (2010) Hum. Genet. 128 (4), 433-441

71565154

NP_000662

NM_000671.4

42.3 kDa (398aa), confirmed by MALDI-TOF

Carbon Metabolism Pathway 814926!!Carbon Metabolism Pathway 817567!!Chemical Carcinogenesis Pathway 673221!!Chemical Carcinogenesis Pathway 673237!!Degradation Of Aromatic Compounds Pathway 843021!!Degradation Of Aromatic Compounds Pathway 717401!!Drug Metabolism - Cytochrome P450 Pathway 83032!!Drug Metabolism - Cytochrome P450 Pathway 427!!Fatty Acid Degradation Pathway 82935!!Fatty Acid Degradation Pathway 296

This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]

0.1 mg

310 USD