VEGF165, 207-371aa, Human, His tag, E Coli | MyBioSource MBS204593 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

VEGF165, 207-371aa, Human, His tag, E Coli

catalog :

MBS204593

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS204593

products type :

Recombinant Protein

products full name :

VEGF165, 207-371aa, Human, His tag, E Coli

products short name :

[VEGF165]

products name syn :

[Vascular endothelial growth factor A isoform d; VPF; VEGF; VEGF-A]

other names :

[vascular endothelial growth factor A isoform d; Vascular endothelial growth factor A; vascular endothelial growth factor A; vascular endothelial growth factor A; Vascular permeability factor; VPF]

products gene name :

[VEGF165]

other gene names :

[VEGFA; VEGFA; VPF; VEGF; MVCD1; VEGF; VEGF-A; VPF]

host :

E Coli

sequence length :

371

sequence :

MGSSHHHHHH SSGLVPRGSH APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

purity :

> 90% by SDS-PAGE

form :

Liquid. In 20mM Tris-HCl (pH 8.5), 50%glycerol, 5mM DTT, 200mM NaCl, 2mM EDTA

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

image1 heading :

SDS-Page

other info1 :

Antigen Species: Human. Tag Information: His-tag

other info2 :

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

products categories :

Hormones & Cytokines

products description :

Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

products references :

Yasuji ueda., et al. (2004) Cancer Chemother Pharmacol. 54 (1):16-24. ; Neufeld G., et al. (1999) FASEB J. 13 (1):9-22.

ncbi gi num :

76781483

ncbi acc num :

NP_001020539

ncbi gb acc num :

NM_001025368.2

uniprot acc num :

P15692

ncbi mol weight :

21.3kDa (185aa), conformed by MALDI-TOF

ncbi pathways :

AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); Allograft Rejection Pathway (920963); Amplification And Expansion Of Oncogenic Pathways As Metastatic Traits (1458200); Angiogenesis Pathway (198772); Aryl Hydrocarbon Receptor Pathway (1458217); Axon Guidance Pathway (1270303); Bladder Cancer Pathway (1458294); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527)

ncbi summary :

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]

uniprot summary :

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development ().

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage