protein

NKIRAS2, 1-191aa, Human, His tag, E Coli

MBS204586

0.05 mg

375 USD

Recombinant Protein

NKIRAS2, 1-191aa, Human, His tag, E Coli

NKIRAS2

NFKB inhibitor interacting Ras-like 2; kappaB-Ras2; KBRAS2

NF-kappa-B inhibitor-interacting Ras-like protein 2 isoform a; NF-kappa-B inhibitor-interacting Ras-like protein 2; NF-kappa-B inhibitor-interacting Ras-like protein 2; I-kappa-B-interacting Ras-like protein 2; NFKB inhibitor interacting Ras-like protein 2; kappa B-Ras protein 2; NFKB inhibitor interacting Ras-like 2; I-kappa-B-interacting Ras-like protein 2; Kappa B-Ras protein 2; KappaB-Ras2

NKIRAS2

NKIRAS2; NKIRAS2; KBRAS2; kappaB-Ras2; KBRAS2; Kappa B-Ras protein 2; KappaB-Ras2

KBRS2_HUMAN

191

MGSSHHHHHH SSGLVPRGSH MGSHMGKSCK VVVCGQASVG KTSILEQLLY GNHVVGSEMI ETQEDIYVGS IETDRGVREQ VRFYDTRGLR DGAELPRHCF SCTDGYVLVY STDSRESFQR VELLKKEIDK SKDKKEVTIV VLGNKCDLQE QRRVDPDVAQ HWAKSEKVKL WEVSVADRRS LLEPFVYLAS KMTQPQSKSA FPLSRKNKGS GSLDG

90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Signaling pathway

NFKB inhibitor interacting Ras-like 2 (NKIRAS2), also known as KBRAS2, is similar to the Ras-like small GTPases that associate with IkappaB. These Ras-like proteins, NKIRAS1 and NKIRAS2, interact with the PEST domains of IkB alpha and IkB beta and decrease their rate of degradation. NKIRAS2 shows 71% identity to KBRAS1. NKIRAS2 is an inhibitor of the transcription factor NF-kappaB. Recombinant human NKIRAS2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

May M J., et al. (1998) Immunol Today. 19:80-88. Bos J L., et al. (1998) Mutat Res. 195:255-271.

222136628

NP_001138399

NM_001144927.1

24.0 kDa (215aa), confirmed by MALDI-TOF

TNF-alpha/NF-kB Signaling Pathway (198884)

NKIRAS2: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF- kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: G protein, monomeric; G protein, monomeric, Ras; G protein. Chromosomal Location of Human Ortholog: 17q21.2. Cellular Component: membrane; cytoplasm. Molecular Function: GTPase activity; GTP binding. Biological Process: I-kappaB kinase/NF-kappaB cascade; metabolic process; small GTPase mediated signal transduction

0.05 mg

375 USD

0.25 mg

925