NPM1, 1-294aa, Human, His tag, Recombinant, E Coli | MyBioSource MBS204570 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

NPM1, 1-294aa, Human, His tag, Recombinant, E Coli

catalog :

MBS204570

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204570

products type :

Recombinant Protein

products full name :

NPM1, 1-294aa, Human, His tag, Recombinant, E Coli

products short name :

NPM1

products name syn :

Nucleophosmin; B23; NPM

other names :

nucleophosmin isoform 1; Nucleophosmin; nucleophosmin; nucleolar protein NO38; nucleophosmin/nucleoplasmin family, member 1; nucleophosmin (nucleolar phosphoprotein B23, numatrin); Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin

products gene name :

NPM1

other gene names :

NPM1; NPM1; B23; NPM; NPM; NPM

uniprot entry name :

NPM_HUMAN

sequence length :

294

sequence :

MGSSHHHHHH SSGLVPRGSH MEDSMDMDMS PLRPQNYLFG CELKADKDYH FKVDNDENEH QLSLRTVSLG AGAKDELHIV EAEAMNYEGS PIKVTLATLK MSVQPTVSLG GFEITPPVVL RLKCGSGPVH ISGQHLVAVE EDAESEDEEE EDVKLLSISG KRSAPGGGSK VPQKKVKLAA DEDDDDDDEE DDDEDDDDDD FDDEEAEEKA PVKKSIRDTP AKNAQKSNQN GKDSKPSSTP RSKGQESFKK QEKTPKTPKG PSSVEDIKAK MQASIEKGGS LPKVEAKFIN YVKNCFRMTD QEAIQDLWQW RKSL

purity :

> 85% by SDS - PAGE

form :

Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.15M NaCl,1mM DTT

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Epigenomics

products description :

NPM1 is a phosphoprotein which moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in NPM1 are associated with acute myeloid leukemia. Recombinant human NPM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Sagawa,F., et al. (2011) EMBO J. 30 (19), 3994-4005. Neubauer,A., et al. (2011) Exp. Hematol. 39 (10), 1030-1042.

ncbi gi num :

10835063

ncbi acc num :

NP_002511

ncbi gb acc num :

NM_002520.6

ncbi mol weight :

34.7kDa (314aa), confirmed by MALDI-TOF

ncbi pathways :

Aurora B Signaling Pathway (138080); BARD1 Signaling Events Pathway (137959); Cell Cycle Pathway (530733); Chromosome Maintenance Pathway (161048); Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway (161050); Disease Pathway (530764); HIF-1-alpha Transcription Factor Network Pathway (138045); HIV Infection Pathway (105983); Host Interactions Of HIV Factors Pathway (106010); Interactions Of Rev With Host Cellular Proteins Pathway (106014)

ncbi summary :

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]

uniprot summary :

NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described. Protein type: RNA-binding; Translation; Nucleolus; Oncoprotein. Chromosomal Location of Human Ortholog: 5q35.1. Cellular Component: nucleoplasm; centrosome; focal adhesion; membrane; cytoplasm; nucleolus; spindle pole centrosome; cytosol; ribonucleoprotein complex; nucleus. Molecular Function: NF-kappaB binding; protein binding; protein homodimerization activity; protein kinase inhibitor activity; histone binding; Tat protein binding; protein heterodimerization activity; RNA binding; unfolded protein binding; transcription coactivator activity; protein kinase binding; ribosomal large subunit binding; ribosomal small subunit binding. Biological Process: regulation of centriole replication; viral reproduction; positive regulation of translation; centrosome cycle; cell aging; nucleocytoplasmic transport; DNA repair; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; intracellular protein transport; nucleosome assembly; regulation of endodeoxyribonuclease activity; protein localization; DNA replication-independent nucleosome assembly at centromere; ribosome assembly; response to stress; negative regulation of apoptosis

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage