MOCS2, 1-188aa, Human, His tag, E Coli | MyBioSource MBS204557 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

MOCS2, 1-188aa, Human, His tag, E Coli

catalog :

MBS204557

quantity :

0.02 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS204557

products type :

Recombinant Protein

products full name :

MOCS2, 1-188aa, Human, His tag, E Coli

products short name :

MOCS2

products name syn :

molybdenum cofactor synthesis 2; MCBPE; MOCO1; MOCS2A; MOCS2B; MPTS

other names :

molybdopterin synthase catalytic subunit large subunit MOCS2B; Molybdopterin synthase catalytic subunit; molybdopterin synthase catalytic subunit; molybdopterin synthase sulfur carrier subunit; MOCS2A; MOCS2B; molybdenum cofactor biosynthesis protein E; molybdopterin synthase small and large subunit; molybdenum cofactor synthesis 2; MOCO1-BMolybdenum cofactor synthesis protein 2 large subunit; Molybdenum cofactor synthesis protein 2B

products gene name :

MOCS2

other gene names :

MOCS2; MOCS2; MPTS; MCBPE; MOCO1; MOCODB

uniprot entry name :

MOC2B_HUMAN

sequence length :

188

sequence :

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSSL EISSSCFSLE TKLPLSPPLV EDSAFEPSRK DMDEVEEKSK DVINFTAEKL SVDEVSQLVI SPLCGAISLF VGTTRNNFEG KKVISLEYEA YLPMAENEVR KICSDIRQKW PVKHIAVFHR LGLVPVSEAS IIIAVSSAHR AASLEAVSYA IDTLKAKVPI WKKEIYEESS TWKGNKECFW ASNS

purity :

> 90% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol,1mM DTT

concentration :

0.25 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Metabolism

products description :

MOCS2 is a heterotetrameric synthase composed of two small (MOCS2A) and two large (MOCS2B) subunits. The large and small subunits of molybdopterin synthase are both encoded from this gene by overlapping open reading frames. MOCS2 functions in the second step of the synthesis of molybdenum cofactor or molybdopterin (MPT). It catalyzes the formation of MPT from precursor Z by incorporating a dithiolene functional group. Recombinant human MOCS2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Leimkuhler S., et al. (2005) Hum Genet. 117: 565-570. Reiss J., et al. (1999) Hum Genet. 64: 706-711.

ncbi gi num :

4758732

ncbi acc num :

NP_004522

ncbi gb acc num :

NM_004531.4

ncbi mol weight :

25 kDa (224aa) confirmed by MALDI-TOF

ncbi pathways :

Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (906010); Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway (906009); Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway (906005)

ncbi summary :

Eukaryotic molybdoenzymes use a unique molybdenum cofactor (MoCo) consisting of a pterin, termed molybdopterin, and the catalytically active metal molybdenum. MoCo is synthesized from precursor Z by the heterodimeric enzyme molybdopterin synthase. The large and small subunits of molybdopterin synthase are both encoded from this gene by overlapping open reading frames. The proteins were initially thought to be encoded from a bicistronic transcript. They are now thought to be encoded from monocistronic transcripts. Alternatively spliced transcripts have been found for this locus that encode the large and small subunits. [provided by RefSeq, Jul 2008]

uniprot summary :

MOCS2: Catalytic subunit of the molybdopterin synthase complex, a complex that catalyzes the conversion of precursor Z into molybdopterin. Acts by mediating the incorporation of 2 sulfur atoms from thiocarboxylated MOCS2A into precursor Z to generate a dithiolene group. Defects in MOCS2 are the cause of molybdenum cofactor deficiency type B (MOCOD type B). MOCOD type B is an autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death. Belongs to the MoaE family. MOCS2B subfamily. Protein type: Enzyme, misc.; EC 2.8.1.12. Chromosomal Location of Human Ortholog: 5q11. Cellular Component: molybdopterin synthase complex; cytoplasm; cytosol; nucleus. Molecular Function: identical protein binding; nucleotide binding; Mo-molybdopterin synthase activity. Biological Process: vitamin metabolic process; Mo-molybdopterin cofactor biosynthetic process; molybdopterin cofactor biosynthetic process; water-soluble vitamin metabolic process. Disease: Molybdenum Cofactor Deficiency, Complementation Group B

size1 :

0.02 mg

price1 :

310 USD

size2 :

0.1 mg

price2 :

720

more info or order :

MyBioSource product webpage