protein

CTH, 1-405aa, Human, His tag, E Coli

MBS204550

0.1 mg

310 USD

Recombinant Protein

CTH, 1-405aa, Human, His tag, E Coli

[CTH]

[Cystathionine gamma-lyase; Cystathionine gamma-lyase; Gamma-cystathionase]

[cystathionine gamma-lyase isoform 1; Cystathionine gamma-lyase; cystathionine gamma-lyase; cystathionine gamma-lyase; Cysteine-protein sulfhydrase; Gamma-cystathionase]

[CTH]

[CTH; CTH]

E Coli

405

MGSSHHHHHH SSGLVPRGSH MQEKDASSQG FLPHFQHFAT QAIHVGQDPE QWTSRAVVPP ISLSTTFKQG APGQHSGFEY SRSGNPTRNC LEKAVAALDG AKYCLAFASG LAATVTITHL LKAGDQIICM DDVYGGTNRY FRQVASEFGL KISFVDCSKI KLLEAAITPE TKLVWIETPT NPTQKVIDIE GCAHIVHKHG DIILVVDNTF MSPYFQRPLA LGADISMYSA TKYMNGHSDV VMGLVSVNCE SLHNRLRFLQ NSLGAVPSPI DCYLCNRGLK TLHVRMEKHF KNGMAVAQFL ESNPWVEKVI YPGLPSHPQH ELVKRQCTGC TGMVTFYIKG TLQHAEIFLK NLKLFTLAES LGGFESLAEL PAIMTHASVL KNDRDVLGIS DTLIRLSVGL EDEEDLLEDL DQALKAAHPP SGSHS

> 95% by SDS-PAGE

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl

1mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

SDS-Page

Antigen Species: Human. Tag Information: His-tag

Metabolism

CTH (Cystathionine gamma-lyase or cystathionase) belongs to the trans-sulfuration enzymes family. This protein is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate. CTH catalyzes the last step in the transsulfuration pathway from methionine to cysteine. Recombinant human CTH protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Chiku T., et al. (2009) J. Biol. Chem. 284:11601-11612; Sun Q., et al. (2009) J. Biol. Chem. 284:3076-3085

21361334

NP_001893

NM_001902.5

P32929

46.7kDa (425aa), confirmed by MALDI-TOF

Amino Acid Metabolism Pathway (1471249); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421); Cysteine Biosynthesis, Homocysteine + Serine = Cysteine Pathway (413414); Cysteine Biosynthesis, Homocysteine + Serine = Cysteine Pathway (468303); Cysteine Formation From Homocysteine Pathway (1270182); Degradation Of Cysteine And Homocysteine Pathway (1270183); Folate Metabolism Pathway (198833)

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.

0.1 mg

310 USD

0.5 mg

720