catalog number :
MBS204514
products type :
Recombinant Protein
products full name :
METAP1, 1-386aa, Human, His tag, E Coli
products short name :
METAP1
products name syn :
methionyl aminopeptidase 1; MAP1A; MetAP1A
other names :
methionine aminopeptidase 1; Methionine aminopeptidase 1; methionine aminopeptidase 1; MAP 1; metAP 1; peptidase M 1; methionyl aminopeptidase 1; Peptidase M 1
products gene name :
METAP1
other gene names :
METAP1; METAP1; MAP1A; MetAP1A; ;
uniprot entry name :
MAP11_HUMAN
sequence :
MGSSHHHHHH SSGLVPRGSH MGSMAAVETR VCETDGCSSE AKLQCPTCIK LGIQGSYFCS QECFKGSWAT HKLLHKKAKD EKAKREVSSW TVEGDINTDP WAGYRYTGKL RPHYPLMPTR PVPSYIQRPD YADHPLGMSE SEQALKGTSQ IKLLSSEDIE GMRLVCRLAR EVLDVAAGMI KPGVTTEEID HAVHLACIAR NCYPSPLNYY NFPKSCCTSV NEVICHGIPD RRPLQEGDIV NVDITLYRNG YHGDLNETFF VGEVDDGARK LVQTTYECLM QAIDAVKPGV RYRELGNIIQ KHAQANGFSV VRSYCGHGIH KLFHTAPNVP HYAKNKAVGV MKSGHVFTIE PMICEGGWQD ETWPDGWTAV TRDGKRSAQF EHTLLVTDTG CEILTRRLDS ARPHFMSQF
purity :
> 90% by SDS - PAGE
form :
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 3mM DTT, 40% glycerol, 200mM NaCl, 0.1mM PMSF
concentration :
1mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Metabolism
products description :
METAP1 (Methionine aminopeptidase 1) belongs to the peptidase M24A family. This protein removes the amino-terminal methionine from nascent proteins. It required for normal progression through the cell cycle. The active site of MetAP-1 contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion. Recombinant human METAP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Dummitt B, et al. (2002) Protein Pept Lett 9 (4): 295-303. Hu X., et al. (2006) Proc. Natl. Acad. Sci. u.S.A. 103:18148-18153.
ncbi gb acc num :
NM_015143.2
ncbi mol weight :
45.7kDa (409aa), confirmed by MALDI-TOF
ncbi pathways :
Disease Pathway 530764!!Diseases Associated With Visual Transduction Pathway 771581!!Inactivation, Recovery And Regulation Of The Phototransduction Cascade Pathway 833795!!Signal Transduction Pathway 477114!!The Phototransduction Cascade Pathway 833793!!Visual Phototransduction Pathway 771584